Mouse Nephronectin Antibody

Catalog # Availability Size / Price Qty
AF4298
AF4298-SP
Nephronectin in Mouse Pancreas.
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Product Details
Citations (2)
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Mouse Nephronectin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Nephronectin in direct ELISAs.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Nephronectin
Asp20-Cys561
Accession # AAH68308
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of mouse pancreas

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Nephronectin in Mouse Pancreas. Nephronectin was detected in immersion fixed paraffin-embedded sections of mouse pancreas using Goat Anti-Mouse Nephronectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4298) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nephronectin

Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70‑90 kDa extracellular matrix protein that is a ligand for integrin alpha 8 beta 1 (1, 2). The 561 amino acid (aa) mouse Nephronectin (isoform b) contains a 19 aa signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2). Isoform a includes a 17 aa insertion between aa 58 and 59 that is missing in isoform b. Mouse Nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, bovine and opossum Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down‑regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in-vivo complex with integrin alpha 8 beta 1 (2). Since deletion of integrin alpha 8 beta 1 results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha 8 beta 1 ligand during Nephrogenesis (2, 3).

References
  1. Morimura, N. et al. (2001) J. Biol. Chem. 276:42172.
  2. Brandenberger, R. et al. (2001) J. Cell Biol. 154:447.
  3. Miner, J. H. (2001) J. Cell Biol. 154:257.
Entrez Gene IDs
255743 (Human); 114249 (Mouse)
Alternate Names
EGFL6L; Nctn; Nephronectin; NPNT; POEM

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Citations for Mouse Nephronectin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nephronectin-integrin alpha8 signaling is required for proper migration of periocular neural crest cells during chick corneal development
    Authors: J Ma, L Bi, J Spurlin, P Lwigale
    Elife, 2022-03-03;11(0):.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Stimulation
  2. A cell-ECM mechanism for connecting the ipsilateral eye to the brain
    Authors: J Su, U Sabbagh, Y Liang, L Olejníková, KG Dixon, AL Russell, J Chen, YA Pan, JW Triplett, MA Fox
    Proceedings of the National Academy of Sciences of the United States of America, 2021-10-19;118(42):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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