Mouse Noggin Antibody

Catalog # Availability Size / Price Qty
AF719
AF719-SP
Noggin in Embryonic Mouse Cardiac Tissue.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (4)

Mouse Noggin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Noggin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Noggin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Noggin
Leu20-Cys232
Accession # P97466
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Noggin (Catalog # 1967-NG)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Noggin antibody in Embryonic Mouse Cardiac Tissue by Immunohistochemistry (IHC-Fr). View Larger

Noggin in Embryonic Mouse Cardiac Tissue. Noggin was detected in immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry Noggin antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC). View Larger

Noggin in PC‑3 Human Cell Line. Noggin was detected in immersion fixed PC-3 human prostate cancer cell line using Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Noggin

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

References
  1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
  2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  3. Brunet, L.J. et al. (1998) Science 280:1455.
Entrez Gene IDs
9241 (Human); 18121 (Mouse)
Alternate Names
NOG; Noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1; SYNS1A

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Citations for Mouse Noggin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Taste papilla cell differentiation requires tongue mesenchyme via ALK3-BMP signaling to regulate the production of secretory proteins
    Authors: M Ishan, Z Wang, P Zhao, Y Yao, S Stice, L Wells, Y Mishina, HX Liu
    bioRxiv : the preprint server for biology, 2023-04-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Myo/Nog Cells Give Rise to Myofibroblasts During Epiretinal Membrane Formation in a Mouse Model of Proliferative Vitreoretinopathy
    Authors: M Crispin, J Gerhart, A Heffer, M Martin, F Abdalla, A Bravo-Nuev, NJ Philp, AE Kuriyan, M George-Wei
    Investigative Ophthalmology & Visual Science, 2023-02-01;64(2):1.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Acute Response and Neuroprotective Role of Myo/Nog Cells Assessed in a Rat Model of Focal Brain Injury
    Authors: S Joseph-Pau, N Morrison, M Braccia, A Payne, L Gugerty, J Mostoller, P Lecker, EJ Tsai, J Kim, M Martin, R Brahmbhatt, G Gorski, J Gerhart, M George-Wei, J Stone, S Purushothu, A Bravo-Nuev
    Frontiers in Neuroscience, 2021-12-07;15(0):780707.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  4. A non-invasive method to generate induced pluripotent stem cells from primate urine
    Authors: J Geuder, LE Wange, A Janjic, J Radmer, P Janssen, JW Bagnoli, S Müller, A Kaul, M Ohnuki, W Enard
    Scientific Reports, 2021-02-10;11(1):3516.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Myo/Nog cells are nonprofessional phagocytes
    Authors: J Gerhart, L Gugerty, P Lecker, F Abdalla, M Martin, O Gerhart, C Gerhart, K Johal, J Bernstein, J Spikes, K Mathers, A Bravo-Nuev, M George-Wei
    PLoS ONE, 2020-08-24;15(8):e0235898.
    Species: Human, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Rhabdomyosarcoma and Wilms tumors contain a subpopulation of noggin producing, myogenic cells immunoreactive for lens beaded filament proteins
    Authors: J Gerhart, K Behling, M Paessler, L Milton, G Bramblett, D Garcia, M Pitts, R Hurtt, M Crawford, R Lackman, D Nguyen, J Infanti, P FitzGerald, M George-Wei
    PLoS ONE, 2019-04-11;14(4):e0214758.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC, IHC-P
  7. Noggin depletion in adipocytes promotes obesity in mice
    Authors: AM Blázquez-M, M Jumabay, P Rajbhandar, T Sallam, Y Guo, J Yao, L Vergnes, K Reue, L Zhang, Y Yao, AM Fogelman, P Tontonoz, AJ Lusis, X Wu, KI Boström
    Mol Metab, 2019-04-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Functional Assay, IHC
  8. Myo/Nog cells are present in the ciliary processes, on the zonule of Zinn and posterior capsule of the lens following cataract surgery
    Authors: J Gerhart, C Withers, C Gerhart, L Werner, N Mamalis, A Bravo-Nuev, V Scheinfeld, P FitzGerald, R Getts, M George-Wei
    Exp. Eye Res., 2018-03-17;171(0):101-105.
    Species: Human, Mouse, Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Myo/Nog cells: targets for preventing the accumulation of skeletal muscle-like cells in the human lens.
    Authors: Gerhart J, Greenbaum M, Scheinfeld V, Fitzgerald P, Crawford M, Bravo-Nuevo A, Pitts M, George-Weinstein M
    PLoS ONE, 2014-04-15;9(4):e95262.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  10. Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors.
    Authors: Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A
    Stem Cells Dev, 2013-02-15;22(9):1342-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. BMP signalling permits population expansion by preventing premature myogenic differentiation in muscle satellite cells.
    Authors: Ono Y, Calhabeu F, Morgan JE
    Cell Death Differ., 2010-08-06;18(2):222-34.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Noggin producing, MyoD-positive cells are crucial for eye development.
    Authors: Gerhart J, Pfautz J, Neely C, Elder J, DuPrey K, Menko AS, Knudsen K, George-Weinstein M
    Dev. Biol., 2009-09-22;336(1):30-41.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  13. MyoD-positive epiblast cells regulate skeletal muscle differentiation in the embryo.
    Authors: Gerhart J, Elder J, Neely C, Schure J, Kvist T, Knudsen K, George-Weinstein M
    J. Cell Biol., 2006-10-23;175(2):283-92.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

Reviews for Mouse Noggin Antibody

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Mouse Noggin Antibody
By Anonymous on 10/15/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Epithelial cells Species: Mouse

Mouse Noggin Antibody
By Anonymous on 07/14/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Macrophages Species: Mouse

Mouse Noggin Antibody
By Anonymous on 07/01/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: fibroblasts Species: Mouse

Mouse Noggin Antibody
By Anonymous on 12/16/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: IC-21 mouse macrophage cell line Species: Mouse