Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Avian - Chicken, Rabbit

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunocytochemistry, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Noggin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Noggin
Leu20-Cys232
Accession # P97466

Specificity

Detects mouse Noggin in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human Noggin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Noggin Antibody

Noggin antibody in Embryonic Mouse Cardiac Tissue by Immunohistochemistry (IHC-Fr).

Noggin in Embryonic Mouse Cardiac Tissue.

Noggin was detected in immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.) using 15 µg/mL Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Noggin antibody in PC-3 Human Cell Line by Immunocytochemistry (ICC).

Noggin in PC‑3 Human Cell Line.

Noggin was detected in immersion fixed PC-3 human prostate cancer cell line using Goat Anti-Mouse Noggin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF719) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Noggin by Immunohistochemistry

Detection of Human Noggin by Immunohistochemistry

Myo/Nog cells contain ink in human tattooed skin.Tissue sections of tattooed skin were double labeled with the G8 mAb and antibodies to Noggin (Nog) (A-D), MyoD (F-I) or alpha -SMA (K-N). The colors of the secondary antibodies are indicated in the photographs. Nuclei were stained with Hoechst dye (blue). Images were produced with the epifluorescence (A-E, J and O) and confocal microscopes (F-I and K-N) with 60x lenses. Overlap of green and red fluorescence appears yellow in merged images (C, D, H, I, M and N). Fluorescent photomicrographs were merged with the corresponding DIC image to visualize the ink (black in D, I and N). Some double labeled cells appeared to contain ink (arrows in D, I and N). All ink laden G8+ cells were alpha -SMA+ (N). Smooth muscle cells of blood vessels also contained alpha -SMA (K). Minimal to no background fluorescence was visible after staining with the anti-goat (E), anti-IgM and anti-IgG (I), and anti-rabbit (O) secondary antibodies only. Bar = 28 μM in E and 5.6 μM in A-D and G-O. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32833999), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Noggin by Immunohistochemistry

Detection of Human Noggin by Immunohistochemistry

Myo/Nog cells contain ink in human tattooed skin.Tissue sections of tattooed skin were double labeled with the G8 mAb and antibodies to Noggin (Nog) (A-D), MyoD (F-I) or alpha -SMA (K-N). The colors of the secondary antibodies are indicated in the photographs. Nuclei were stained with Hoechst dye (blue). Images were produced with the epifluorescence (A-E, J and O) and confocal microscopes (F-I and K-N) with 60x lenses. Overlap of green and red fluorescence appears yellow in merged images (C, D, H, I, M and N). Fluorescent photomicrographs were merged with the corresponding DIC image to visualize the ink (black in D, I and N). Some double labeled cells appeared to contain ink (arrows in D, I and N). All ink laden G8+ cells were alpha -SMA+ (N). Smooth muscle cells of blood vessels also contained alpha -SMA (K). Minimal to no background fluorescence was visible after staining with the anti-goat (E), anti-IgM and anti-IgG (I), and anti-rabbit (O) secondary antibodies only. Bar = 28 μM in E and 5.6 μM in A-D and G-O. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32833999), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Noggin by Immunohistochemistry

Detection of Human Noggin by Immunohistochemistry

Myo/Nog cells contain ink in human tattooed skin.Tissue sections of tattooed skin were double labeled with the G8 mAb and antibodies to Noggin (Nog) (A-D), MyoD (F-I) or alpha -SMA (K-N). The colors of the secondary antibodies are indicated in the photographs. Nuclei were stained with Hoechst dye (blue). Images were produced with the epifluorescence (A-E, J and O) and confocal microscopes (F-I and K-N) with 60x lenses. Overlap of green and red fluorescence appears yellow in merged images (C, D, H, I, M and N). Fluorescent photomicrographs were merged with the corresponding DIC image to visualize the ink (black in D, I and N). Some double labeled cells appeared to contain ink (arrows in D, I and N). All ink laden G8+ cells were alpha -SMA+ (N). Smooth muscle cells of blood vessels also contained alpha -SMA (K). Minimal to no background fluorescence was visible after staining with the anti-goat (E), anti-IgM and anti-IgG (I), and anti-rabbit (O) secondary antibodies only. Bar = 28 μM in E and 5.6 μM in A-D and G-O. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32833999), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Noggin Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse cardiac tissue (11 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Noggin (Catalog # 1967-NG)

Reviewed Applications

Read 5 reviews rated 4.8 using AF719 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Noggin

Noggin was originally cloned based on its dorsalizing activity in Xenopus embryos. Mammalian Noggins were subsequently identified and cloned from human, mouse and rat cDNA libraries. Mouse Noggin cDNA encodes a 232 amino acid (aa) residue precursor protein with 19 aa residue putative signal peptide that is cleaved to generate the 213 aa residue mature protein which is secreted as a homodimeric glycoprotein. Noggin is a highly conserved molecule. Mature mouse Noggin shares 99% and 83% aa sequence identity with human and Xenopus Noggin, respectively. Noggin has a complex pattern of expression during embryogenesis. In the adult, Noggin is expressed in the central nervous system and in several adult peripheral tissues such as lung, skeletal muscle and skin. Noggin has been shown to be a high-affinity BMP (bone morphogenetic protein) binding protein that antagonizes BMP bioactivities.

References

  1. Smith, W.C. and R.M. Harland (1992) Cell 70:829.
  2. Valenzuela, D.M. et al. (1995) J. Neurosci. 15:6077.
  3. Brunet, L.J. et al. (1998) Science 280:1455.

Alternate Names

NOG, SYM1, SYNS1, SYNS1A

Entrez Gene IDs

9241 (Human); 18121 (Mouse)

Gene Symbol

NOG

UniProt

P97466

Additional Noggin Products

Product Documents for Mouse Noggin Antibody

Certificate of Analysis

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Product Specific Notices for Mouse Noggin Antibody

For research use only

Citations for Mouse Noggin Antibody

Customer Reviews for Mouse Noggin Antibody (5)

4.8 out of 5
5 Customer Ratings
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Customer Images

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  • Mouse Noggin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Epithelial cells
    Species: Mouse
    Verified Customer | Posted 10/15/2021
    Mouse Noggin Antibody AF719
  • Mouse Noggin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Macrophages
    Species: Mouse
    Verified Customer | Posted 07/14/2021
    Mouse Noggin Antibody AF719
  • Mouse Noggin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: fibroblasts
    Species: Mouse
    Verified Customer | Posted 07/01/2021
    Mouse Noggin Antibody AF719
  • Mouse Noggin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: IC-21 mouse macrophage cell line
    Species: Mouse
    Verified Customer | Posted 12/16/2020
    Mouse Noggin Antibody AF719
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24042462
    Species: Mouse
    Verified Customer | Posted 01/09/2015

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