Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2096C
View all PD-L1/B7-H1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1
Met1-His239
Accession # Q9EP73

Specificity

Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse PD-L1/B7-H1 Antibody

Detection of Mouse PD-L1/B7-H1 antibody by Western Blot.

Detection of Mouse PD-L1/B7-H1 by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of PD-L1/B7-H1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of PD-L1/B7-H1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Detection of PD-L1/B7-H1 antibody in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP antibody by Flow Cytometry.

Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

PD-L1/B7-H1 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr).

PD-L1/B7-H1 in Mouse Thymus.

PD-L1/B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse PD-L1/B7-H1 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS and HEK293 human embryonic kidney cell line transfected with mouse B7-H1/PD-L1

Immunohistochemistry

5-25 µg/mL
Sample:

Perfusion fixed frozen sections of mouse thymus

Western Blot

2 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line treated with LPS

Reviewed Applications

Read 10 reviews rated 4.4 using MAB90781 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PD-L1/B7-H1

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium
(1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also beenreported (1-4).

References

  1. Tamura, H. et al. (2001) Blood 97:1809.
  2. Freeman, G. et al. (2000) J. Exp. Med. 192:1027.
  3. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9EP73

Additional PD-L1/B7-H1 Products

Product Documents for Mouse PD-L1/B7-H1 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse PD-L1/B7-H1 Antibody

For research use only

Citations for Mouse PD-L1/B7-H1 Antibody

Customer Reviews for Mouse PD-L1/B7-H1 Antibody (10)

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Customer Images

Showing  1 - 5 of 10 reviews Showing All
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  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line
    Species: Mouse
    Verified Customer | Posted 09/26/2021
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse osteosarcoma cell line
    Species: Mouse
    Verified Customer | Posted 06/08/2020
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Breast cancer tissue
    Species: Mouse
    Verified Customer | Posted 04/03/2020
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mouse Breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 12/09/2019
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Dong Hu
    Application: Western Blot
    Sample Tested: mouse breast cancer cell line Eo771
    Species: Mouse
    Verified Customer | Posted 02/28/2019
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: 4T1 mouse breast cancer cell line and B16-F10 mouse melanoma cell line
    Species: Mouse
    Verified Customer | Posted 10/28/2018
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EMT6 cells and CT26
    Species: Mouse
    Verified Customer | Posted 10/17/2018
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Ana Sofia Mendes Leal
    Application: Western Blot
    Sample Tested: Mammary gland tissue and Adenocarcinoma
    Species: Mouse
    Verified Customer | Posted 04/13/2018
    Tissue lysates from mammary gland tumors
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse PD-L1/B7-H1 Antibody
    Name: Mozhdeh Sojoodi
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Pancreatic cancer tissue
    Species: Mouse
    Verified Customer | Posted 08/08/2017
    1:50 concentration, Overnight incubation
    Mouse PD-L1/B7-H1 Antibody MAB90781
  • Mouse B7-H1/PD-L1 Antibody
    Name: Emily Ehlerding
    Application: Immunocytochemistry
    Sample Tested: Mouse Lymph Node
    Species: Mouse
    Verified Customer | Posted 05/16/2017
    Mouse PD-L1/B7-H1 Antibody MAB90781

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