Mouse PD-L1/B7-H1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB90781-100
MAB90781-SP
Detection of Mouse PD-L1/B7-H1 by Western Blot.
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Product Details
Citations (9)
FAQs
Supplemental Products
Reviews (8)

Mouse PD-L1/B7-H1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PD-L1/B7-H1 in direct ELISAs and Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2096C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PD-L1/B7-H1
Met1-His239
Accession # Q9EP73
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse PD-L1/B7-H1 antibody by Western Blot. View Larger

Detection of Mouse PD-L1/B7-H1 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and J774A.1 mouse reticulum cell sarcoma macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of PD-L1/B7-H1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line either treated with LPS overnight (filled histogram) or untreated (open histogram) was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of PD-L1/B7-H1 antibody in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP antibody by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Mouse PD-L1/B7-H1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse PD-L1/B7-H1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry PD-L1/B7-H1 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

PD-L1/B7-H1 in Mouse Thymus. PD-L1/B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using Rabbit Anti-Mouse PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB90781) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to thymocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Mouse B7 homolog 1(B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance (1-4). Other family members include B7-1, B7-2, B7-H2, B7-H3 and PD-L2. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and a short cytoplasmic region. Among the family members, they share from 20-40% amino acid (aa) sequence identity. The cloned mouse B7-H1/PD-L1 cDNA encodes a 290 aa type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular region containing one V-like and one C-like Ig domain, a 22 aa transmembrane region, and a 30 aa cytoplasmic domain. Mouse and human B7-H1/PD-L1 share approximately 70% aa sequence identity. B7-H1/PD-L1 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The other identified ligand is PD-L2. Mouse B7-H1/PD-L1 and PD-L2 share approximately 34% aa sequence identity and have similar functions. B7-H1/PD-L1 is constitutively expressed in various lymphoid and non-lymphoid organs including placenta, heart, pancreas, lung, liver, and endothelium
(1‑4). The expression of B7-H1/PD-L1 is detected on B cells, T cells, monocytes, dendritic cells and thymic epithelial cells. IFN-gamma treatment induces B7-H1/PD-L1 expression in monocytes, dendritic cells, and endothelial cells. B7-H1/PD-L1 expression is also upregulated in a variety of tumor cell lines. On previously activated T cells, B7-H1/PD-L1 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a costimulatory function for the PD-1 ligands on resting T cells has also beenreported (1-4).

References
  1. Tamura, H. et al. (2001) Blood 97:1809.
  2. Freeman, G. et al. (2000) J. Exp. Med. 192:1027.
  3. Sharpe, A.H. and G. J. Freeman (2002) Nat. Rev. Immunol. 2:116.
  4. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

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Citations for Mouse PD-L1/B7-H1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Enhanced bypass of PD-L1 translation reduces the therapeutic response to mTOR kinase inhibitors
    Authors: Cao, Y;Ye, Q;Ma, M;She, QB;
    Cell reports
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. CDK4/6 inhibitors and the pRB-E2F1 axis suppress PVR and PD-L1 expression in triple-negative breast cancer
    Authors: Shrestha, M;Wang, DY;Ben-David, Y;Zacksenhaus, E;
    Oncogenesis
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. PD-L1 induction via the MEK-JNK-AP1 axis by a neddylation inhibitor promotes cancer-associated immunosuppression
    Authors: S Zhang, X You, T Xu, Q Chen, H Li, L Dou, Y Sun, X Xiong, MA Meredith, Y Sun
    Cell Death & Disease, 2022-10-03;13(10):844.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  4. Engineered immunomodulatory accessory cells improve experimental allogeneic islet transplantation without immunosuppression
    Authors: X Wang, K Wang, M Yu, D Velluto, X Hong, B Wang, A Chiu, JM Melero-Mar, AA Tomei, M Ma
    Science Advances, 2022-07-22;8(29):eabn0071.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Colorectal Cancer Progression Is Potently Reduced by a Glucose-Free, High-Protein Diet: Comparison to Anti-EGFR Therapy
    Authors: K Skibbe, AK Brethack, A Sünderhauf, M Ragab, A Raschdorf, M Hicken, H Schlichtin, J Preira, J Brandt, D Castven, B Föh, R Pagel, JU Marquardt, C Sina, S Derer
    Cancers, 2021-11-19;13(22):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The role of PD-1/PD-L1 checkpoint in arsenic lung tumorigenesis
    Authors: W Xu, J Cui, L Wu, C He, G Chen
    Toxicology and Applied Pharmacology, 2021-06-22;426(0):115633.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Knockdown of CDK5 down-regulates PD-L1 via the ubiquitination-proteasome pathway and improves antitumor immunity in lung adenocarcinoma
    Authors: L Gao, L Xia, W Ji, Y Zhang, W Xia, S Lu
    Translational Oncology, 2021-06-12;14(9):101148.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Human Biofield Therapy and the Growth of Mouse Lung Carcinoma
    Authors: P Yang, Y Jiang, PR Rhea, T Coway, D Chen, M Gagea, SL Harribance, L Cohen
    Integr Cancer Ther, 2019-01-01;18(0):1534735419840.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. The Circadian Clock Controls Immune Checkpoint Pathway in Sepsis
    Authors: W Deng, S Zhu, L Zeng, J Liu, R Kang, M Yang, L Cao, H Wang, TR Billiar, J Jiang, M Xie, D Tang
    Cell Rep, 2018-07-10;24(2):366-378.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Mouse PD-L1/B7-H1 Antibody

Average Rating: 4.4 (Based on 8 Reviews)

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Mouse PD-L1/B7-H1 Antibody
By Anonymous on 09/26/2021
Application: WB Sample Tested: J774A.1 mouse reticulum cell sarcoma macrophage cell line Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Anonymous on 06/08/2020
Application: WB Sample Tested: Mouse osteosarcoma cell line Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Anonymous on 12/09/2019
Application: WB Sample Tested: Mouse Breast cancer cell line Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Dong Hu on 02/28/2019
Application: WB Sample Tested: mouse breast cancer cell line Eo771 Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Anonymous on 10/28/2018
Application: WB Sample Tested: 4T1 mouse breast cancer cell line,B16-F10 mouse melanoma cell line Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Anonymous on 10/17/2018
Application: WB Sample Tested: CT26,EMT6 cells Species: Mouse

Mouse PD-L1/B7-H1 Antibody
By Ana Sofia Mendes Leal on 04/13/2018
Application: WB Sample Tested: Adenocarcinoma,Mammary gland tissue Species: Mouse

Tissue lysates from mammary gland tumors


Mouse PD-L1/B7-H1 Antibody
By Mozhdeh Sojoodi on 08/08/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Pancreatic cancer tissue Species: Mouse

1:50 concentration, Overnight incubation