Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antibody

(1 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse CD47 N-terminal IgV-like in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human CD47 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse CD47 N-terminal IgV-like
    Gln19-Pro158
    Accession # NP_034711
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25-1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse ovary and thymus
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CD47 by Western Blot. Western blot shows lysates of mouse ovary tissue and mouse thymus tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse/Rat CD47 N-terminal IgV-like Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1866) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for CD47 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Rat CD47 by Western Blot. Western blot shows lysates of rat placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat CD47
N-terminal IgV-like Extracellular Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1866) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for CD47 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD47
CD47, also known as Integrin‑Associated Protein (IAP) and OA3, is a 40‑60 kDa variably glycosylated atypical member of the immunoglobulin superfamily (1, 2). Mouse CD47 is an integral membrane protein that consists of a 122 amino acid (aa) extracellular domain (ECD) with a single Ig‑like domain, five membrane-spanning regions with short intervening loops, and a 16 aa C‑terminal cytoplasmic tail (3). Alternate splicing of mouse CD47 generates an additional isoform with an insertion of 21 aa following the Ig‑like domain (3). Within the N‑terminal ECD, mouse CD47 shares 63% and 84% aa sequence identity with human and rat CD47, respectively. A portion of the N‑terminal ECD can by shed from smooth muscle cells by MMP-2‑mediated proteolysis (4). The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells (5, 6). These interactions prevent macrophage‑mediated clearance of healthy CD47-expressing cells and promote immune cell transmigration across the vascular endothelium (5‑8). CD47 associates in cis with Fas on T cells and enhances Fas‑mediated apoptosis; its ligation promotes T cell anergy and dampens Th1 immune responses (9‑11). CD47 also associates in cis with Integrins alpha 4 beta 1, alpha V beta 3, alpha 2b beta 3, and alpha 2 beta 1 which can positively or negatively modulate Integrin-mediated function (2, 12). In the vasculature, CD47 binding by Thrombospondin‑1 inhibits the angiogenic and vasorelaxant effects of nitric oxide (2, 13, 14). On dendritic cells and myeloma cells, CD47 ligation by TSP‑1 induces giant cell formation and osteoclast differentiation (15).
  • References:
    1. Sarfati, M. et al. (2009) Curr. Drug Targ. 9:842.
    2. Isenberg, J.S. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:615.
    3. Lindberg, F.P. et al. (1993) J. Cell Biol. 123:485.
    4. Maile, L.A. et al. (2008) Mol. Endocrinol. 22:1226.
    5. Oldenborg, P.-A. et al. (2000) Science 288:2051.
    6. Liu, Y. et al. (2002) J. Biol. Chem. 277:10028.
    7. Stefanidakis, M. et al. (2008) Blood 112:1280.
    8. de Vries, H.E. et al. (2002) J. Immunol. 168:5832.
    9. Manna, P.P. et al. (2005) J. Biol. Chem. 280:29637.
    10. Avice, M.-N. et al. (2001) J. Immunol. 167:2459.
    11. Bouguermouh, S. et al. (2008) J. Immunol. 180:8073.
    12. Barazi, H.O. et al. (2002) J. Biol. Chem. 277:42859.
    13. Isenberg, J.S. et al. (2009) Nitric Oxide 21:52.
    14. Isenberg, J.S. et al. (2009) Matrix Biol. 28:110.
    15. Kukreja, A. et al. (2009) Blood 114:3413.
  • Entrez Gene IDs:
    961 (Human); 16423 (Mouse); 102139846 (Cynomolgus Monkey)
  • Alternate Names:
    antigen identified by monoclonal 1D8; Antigenic surface determinant protein OA3; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47; IAP; IAPintegrin associated protein; Integrin-associated protein; leukocyte surface antigen CD47; MER6integrin-associated signal transducer; OA3; Protein MER6; Rh-related antigen
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Radiation-induced loss of cell surface CD47 enhances immune-mediated clearance of human papillomavirus-positive cancer.
    Authors: Vermeer D, Spanos W, Vermeer P, Bruns A, Lee K, Lee J
    Int J Cancer, 2013;133(1):120-9.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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