Mouse/Rat MyD88 Antibody

Catalog # Availability Size / Price Qty
AF3109
AF3109-SP
Detection of Mouse/Rat MyD88 by Western Blot.
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Product Details
Citations (8)
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Mouse/Rat MyD88 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat MyD88 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse MyD88
Met1-Pro296
Accession # Q3U7M4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat MyD88 antibody by Western Blot. View Larger

Detection of Mouse/Rat MyD88 by Western Blot. Western blot shows lysates of L6 rat myoblast cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MyD88 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry MyD88 antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

MyD88 in RAW 264.7 Mouse Cell Line. MyD88 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of MyD88 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of MyD88 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. This application has not been tested in rat samples.

Simple Western Detection of Rat MyD88 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat MyD88 by Simple WesternTM. Simple Western lane view shows lysates of Nb2-11 rat lymphoma cell line, L6 rat myoblast cell line, and NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MyD88

Myeloid Differentiation primary response protein 88 (MyD88) is a 296 amino acid, 34 kDa, ubiquitously expressed, cytoplasmic adaptor protein involved in the signaling of TLR and IL-1 R family members. MyD88 contains an N-terminal death domain and a C-terminal Toll/IL-1 R (TIR) domain. Each domain seems to participate in protein-protein interactions, as the death domain is inactive. Upon Toll receptor ligation, MyD88 is recruited to the receptor and initiates a signaling cascade that results in NK kappa B and JNK activation. The amino acid sequence of mouse MyD88 is 93% identical to that of rat MyD88.

Long Name
Myeloid Differentiation Primary Response Gene 88
Entrez Gene IDs
4615 (Human); 17874 (Mouse)
Alternate Names
MyD88; MYD88D; myeloid differentiation primary response gene (88); myeloid differentiation primary response protein MyD88

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Citations for Mouse/Rat MyD88 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. The IRAK4 scaffold integrates TLR4-driven TRIF and MYD88 signaling pathways
    Authors: M Pereira, DF Durso, CE Bryant, EA Kurt-Jones, N Silverman, DT Golenbock, RT Gazzinelli
    Cell Reports, 2022-08-16;40(7):111225.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Immunoprecipitation, Western Blot
  2. IL-33/ST2 receptor-dependent signaling in the development of pulmonary hypertension in Sugen/hypoxia mice
    Authors: CS Indralinga, AK Gutierrez-, SC Johns, T Tsui, DT Cannon, MM Fuster, TD Bigby, PA Jennings, EC Breen
    Physiological Reports, 2022-02-01;10(3):e15185.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Macrophage activation on "phagocytic synapse" arrays: Spacing of nanoclustered ligands directs TLR1/2 signaling with an intrinsic limit
    Authors: M Li, H Wang, W Li, XG Xu, Y Yu
    Science Advances, 2020-12-02;6(49):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. The Single Nucleotide Polymorphism Mal-D96N Mice Provide New Insights into Functionality of Mal in TLR Immune Responses
    Authors: JK Dowling, ME Tate, NM Bourke, N Bitto, MA Lauterbach, S Cheung, T Ve, B Kobe, D Golenbock, A Mansell
    J. Immunol., 2019-02-20;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Immunoprecipitation, Proximity Ligation Assay (PLA)
  5. Interleukin 1 receptor-associated kinase 4 (IRAK4) plays a dual role in Myddosome formation and Toll-like receptor signalling
    Authors: D De Nardo, KR Balka, Y Cardona Gl, VR Rao, E Latz, SL Masters
    J. Biol. Chem., 2018-08-03;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Blocking TIR Domain Interactions in TLR9 Signaling
    Authors: A Javmen, H Szmacinski, JR Lakowicz, VY Toshchakov
    J. Immunol., 2018-06-18;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  7. Signaling through the adaptor molecule MyD88 in CD4+ T cells is required to overcome suppression by regulatory T cells.
    Authors: Schenten D, Nish S, Yu S, Yan X, Lee H, Brodsky I, Pasman L, Yordy B, Wunderlich F, Bruning J, Zhao H, Medzhitov R
    Immunity, 2014-01-16;40(1):78-90.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages.
    Authors: Cole LE, Shirey KA, Barry E, Santiago A, Rallabhandi P, Elkins KL, Puche AC, Michalek SM, Vogel SN
    Infect. Immun., 2007-05-21;75(8):4127-37.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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