Mouse/Rat MyD88 Antibody

Detection of Rat MyD88 by Simple Western^TM.

Simple Western lane view shows lysates of Nb2-11 rat lymphoma cell line, L6 rat myoblast cell line, and NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for MyD88 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat MyD88 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3109) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse Mouse/Rat MyD88 Antibody by Western Blot

Examination of IRAK1 in myddosome interactions. D, WT iBMDMs expressing IRAK1-mCerulean were left untreated or stimulated with 1 μg/ml LPS for 30 min or 2 h. WCLs were then subjected to GFP IP followed by immunoblotting for MyD88 and GFP or directly subjected to immunoblotting with the same antibodies. MyD88 in WCLs also served as a loading control. The data presented are representative of three independent experiments.Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30076215), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Mouse/Rat MyD88 Antibody by Western Blot

Examination of IRAK1 in myddosome interactions.A and B, WT iBMDMs expressing IRAK1-mCerulean were left untreated (UT) or stimulated with 1 μg/ml LPS for 2 or 4 h. E, WT iBMDMs expressing IRAK1-mCerulean were pretreated with either DMSO or 20 μm I4 inhb for 30 min. Cells were then left untreated or stimulated with 1 μg/ml LPS for a further 30 min. WCLs were then subjected to GFP IP followed by immunoblotting with MyD88 and GFP or directly subjected to immunoblotting for GFP, P-IRAK4, and MyD88. The data presented are representative of four independent experiments. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30076215), licensed under a CC-BY license. Not internally tested by R&D Systems.