Mouse/Rat Neuropilin-2 Antibody

Catalog # Availability Size / Price Qty
AF567
AF567-SP
Mouse/Rat Neuropilin-2 Antibody in Data
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Product Details
Citations (12)
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Reviews (3)

Mouse/Rat Neuropilin-2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Neuropilin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human Neuropilin-2 and recombinant rat Neuropilin-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2.5-15 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human VEGF165 (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-2 Fc Chimera (Catalog # 567-N2) coated at 5 µg/mL (100 µL/well). At 60 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse and Rat Neuropilin-2 antibody by Western Blot. View Larger

Detection of Mouse and Rat Neuropilin‑2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neuropilin-2 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Neuropilin‑2 in Rat Brain. Neuropilin-2 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse and Rat Neuropilin-2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse and Rat Neuropilin‑2 by Simple WesternTM. Simple Western lane view shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin-2 at approximately 140 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as Neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely nonoverlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn‑1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

References
  1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
  2. Neufeld, G. et al. (1999) FASEB J. 13:9.
  3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.
Entrez Gene IDs
8828 (Human); 18187 (Mouse); 81527 (Rat)
Alternate Names
MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

Product Datasheets

Citations for Mouse/Rat Neuropilin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
    Authors: M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen
    Nat Commun, 2018;9(1):1511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction
    Authors: F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi
    JCI Insight, 2017;2(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins
    Authors: MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen
    Genesis, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis
    Authors: Rainer H Straub
    J. Med. Microbiol., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
    Authors: Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P
    J Neurosci, 2014;34(34):11274-87.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  7. Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
    Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D
    Am J Pathol, 2014;184(5):1577-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
    Authors: Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M
    Dev Biol, 2014;386(1):25-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  9. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010;120(5):1694-707.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  10. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
    Authors: Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
    J. Clin. Invest., 2008;118(6):2062-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. BIG-2 mediates olfactory axon convergence to target glomeruli.
    Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
    Neuron, 2008;57(6):834-46.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.
    Authors: Ito K, Kawasaki T, Takashima S, Matsuda I, Aiba A, Hirata T
    J. Neurosci., 2008;28(17):4414-22.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Mouse/Rat Neuropilin-2 Antibody

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Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 12/03/2020
Application: ICC/IF Sample Tested: E12.5 mouse embryo fixed in 4% PFA Species: Mouse

Antibody was stained on E12.5 mouse sections (attached picture) as well as E9.5 mouse sections.
Worked well.
Note the positive stain in the veins and the negative signal in the adjacent arteries.
Dilution used - 1:20


Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 11/08/2016
Application: IHC Sample Tested: Embryonic tissue Species: Mouse

Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 09/07/2016
Application: Whole mount immunofluorescence Sample Tested: Whole mount eye tissue Species: Mouse

Whole mouse eye tissue was fixed in 2% PFA, blocked and incubated O/N in AF567 diluted 1:250. Signal was detected using an alexafluor 488-labeled donkey anti goat secondary