Mouse/Rat Neuropilin-2 Antibody

(10 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat Neuropilin-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human Neuropilin-2 is observed, and less than 5% cross-reactivity with recombinant rat Neuropilin-1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
    Gln23-Asp857 (Val809-Asp825 del)
    Accession # O35276
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-3 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human VEGF165 (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-2 Fc Chimera (Catalog # 567-N2) coated at 5 µg/mL (100 µL/well). At 60 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse and Rat Neuropilin‑2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuropilin‑2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Neuropilin‑2 in Rat Brain. Neuropilin‑2 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Detection of Mouse and Rat Neuropilin‑2 by Simple WesternTM. Simple Western lane view shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin‑2 at approximately 140 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat Neuropilin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as Neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely nonoverlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn‑1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

  • References:
    1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
    2. Neufeld, G. et al. (1999) FASEB J. 13:9.
    3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.
  • Entrez Gene IDs:
    8828 (Human); 18187 (Mouse); 81527 (Rat)
  • Alternate Names:
    MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
Filter your results:

Species
Applications
Sample Type
  1. Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction
    Authors: F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi
    JCI Insight, 2017;2(12):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins
    Authors: MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen
    Genesis, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016;30(12):1454-69.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
    Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D
    Am J Pathol, 2014;184(5):1577-92.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
    Authors: Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P
    J Neurosci, 2014;34(34):11274-87.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Immunopreciptation
  6. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
    Authors: Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M
    Dev Biol, 2014;386(1):25-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010;120(5):1694-707.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: WB
  8. Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.
    Authors: Ito K, Kawasaki T, Takashima S, Matsuda I, Aiba A, Hirata T
    J. Neurosci., 2008;28(17):4414-22.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  9. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
    Authors: Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
    J. Clin. Invest., 2008;118(6):2062-75.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. BIG-2 mediates olfactory axon convergence to target glomeruli.
    Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
    Neuron, 2008;57(6):834-46.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Immunohistochemistry Reagents
Description Application Cat# Citations Images  

Antigen Retrieval Reagent-Universal

CTS015 10
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Basic

CTS013 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mounting Medium

CTS011 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Antigen Retrieval Reagent-Acidic

CTS014
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

NorthernLights Guard Mounting Media

NL996
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG HRP-conjugated Antibody

WB HAF017 15  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Antibody

WB AF109 6
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG VisUCyte HRP Polymer

IHC VC004  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 5 (Based on 2 reviews)

Have you used Mouse/Rat Neuropilin-2 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Mouse/Rat Neuropilin-2 Antibody.

We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Whole mount immunofluorescence.

Filter by Ratings
Excellent
(2)
All Ratings
Filter by Applications
IHC
(1)
Whole mount immunofluorescence
(1)
All Applications
Filter by Species
Mouse
(2)
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse/Rat Neuropilin-2 Antibody AF567
Enlarge
Excellent
 IHC Mouse Anonymous 11/08/2016
Show
Image Details
Immunohistochemistry Mouse/Rat Neuropilin-2 Antibody AF567
Immunohistochemistry: Mouse/Rat Neuropilin-2 Antibody [AF567]

Summary

ApplicationImmunohistochemistry
Sample TestedEmbryonic tissue
SpeciesMouse
Whole mount immunofluorescence Mouse/Rat Neuropilin-2 Antibody AF567
Enlarge
Excellent
 Whole mount immunofluorescence Mouse Anonymous 09/07/2016
Show
Image Details
Whole mount immunofluorescence Mouse/Rat Neuropilin-2 Antibody AF567
Whole mount immunofluorescence: Mouse/Rat Neuropilin-2 Antibody [AF567]

Summary

ApplicationWhole mount immunofluorescence
Sample TestedWhole mount eye tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsWhole mouse eye tissue was fixed in 2% PFA, blocked and incubated O/N in AF567 diluted 1:250. Signal was detected using an alexafluor 488-labeled donkey anti goat secondary

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information