Mouse SCF/c-kit Ligand Antibody Summary
Accession # Q78ED8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by SCF/c‑kit Ligand and Neutralization by Mouse SCF/c‑kit Ligand Antibody.
Recombinant Mouse SCF/
c‑kit Ligand (Catalog # 455‑MC) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c‑kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/
c-kit Ligand Polyclonal Antibody (Catalog # AB-455-NA). The ND50 is typically 4-20 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCF/c-kit Ligand
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28-40 kDa type I transmembrane glycoprotein (1). It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1-7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail (9). The ECD shows both N-linked and O-linked glycosylation (10). Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice (11, 12). An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site (13). Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72-75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (14). Non-covalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling (15). SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (16).
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- Kapur, R. et al. (2002) Blood 100:1287.
- Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540.
- Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039.
- Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
- Huang, E. et al. (1990) Cell 63:225.
- Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
- Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
- Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4671.
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- Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.
- Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
Citation for Mouse SCF/c-kit Ligand Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Adult mouse myometrial label-retaining cells divide in response to gonadotropin stimulation.
Authors: Szotek PP, Chang HL, Zhang L, Preffer F, Dombkowski D, Donahoe PK, Teixeira J
Stem Cells, 2007;25(5):1317-25.
Sample Types: Whole Tissue
Applications: IHC Paraffin-embedded
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