Mouse Semaphorin 6A Antibody

Catalog #: AF1615
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1615
AF1615-SP

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Detection of Semaphorin 6A in Mouse Intestine.

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Citations (3)

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Mouse Semaphorin 6A Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Semaphorin 6A is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3F, and rmSemaphorin 7A is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Semaphorin 6A

Immunohistochemistry

1-15 µg/mL

Immersion fixed paraffin-embedded sections of Mouse Intestine

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Detection of Semaphorin 6A in Mouse Intestine. Semaphorin 6A was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Semaphorin 6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1615) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal (1).

References

Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.

Entrez Gene IDs

57556 (Human); 20358 (Mouse)

Alternate Names

KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Mouse Semaphorin 6A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
Authors: Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A
Arterioscler Thromb Vasc Biol, 2014;34(0):99.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Semaphorin 6A regulates angiogenesis by modulating VEGF signaling.
Authors: Segarra, Marta, Ohnuki, Hidetaka, Maric, Dragan, Salvucci, Ombretta, Hou, Xu, Kumar, Anil, Li, Xuri, Tosato, Giovanna
Blood, 2012;120(19):4104-15.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Cis interaction between Semaphorin6A and Plexin-A4 modulates the repulsive response to Sema6A.
Authors: Haklai-Topper L, Mlechkovich G, Savariego D
EMBO J., 2010;29(15):2635-45.
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: ICC