Mouse Semaphorin 6A Antibody

Catalog # Availability Size / Price Qty
AF1615
AF1615-SP
Product Details
Citations (3)
FAQs
Supplemental Products
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Mouse Semaphorin 6A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Semaphorin 6A is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3F, and rmSemaphorin 7A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Semaphorin 6A
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (forebrain) and mouse embryo (E13.5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal (1).

References
  1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.
Entrez Gene IDs
57556 (Human); 20358 (Mouse)
Alternate Names
KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

Product Datasheets

Citations for Mouse Semaphorin 6A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
    Authors: Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A
    Arterioscler Thromb Vasc Biol, 2014;34(0):99.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Semaphorin 6A regulates angiogenesis by modulating VEGF signaling.
    Authors: Segarra, Marta, Ohnuki, Hidetaka, Maric, Dragan, Salvucci, Ombretta, Hou, Xu, Kumar, Anil, Li, Xuri, Tosato, Giovanna
    Blood, 2012;120(19):4104-15.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Cis interaction between Semaphorin6A and Plexin-A4 modulates the repulsive response to Sema6A.
    Authors: Haklai-Topper L, Mlechkovich G, Savariego D
    EMBO J., 2010;29(15):2635-45.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: ICC

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