Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TIM-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2
His22-Asn229
Accession # NP_599010

Specificity

Detects mouse TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human TIM-1 and recombinant mouse TIM-3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TIM‑2 Antibody

TIM-2 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr).

TIM‑2 in Mouse Liver.

TIM-2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse TIM-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1885) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse TIM‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse liver

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TIM‑2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIM-2

TIM-2 is a type I membrane protein and is a member of the TIM protein family. This protein family is characterized by their conserved immunoglobulin and mucin domains, as well as by their expression on activated T cells. TIM-2 is a receptor for Sema 4A, which is expressed on dendritic cells and B cells. Sema 4A has been shown to enhance T cell activation.

Long Name

T Cell Immunoglobulin Mucin-2

Alternate Names

TIM2

Entrez Gene IDs

171284 (Mouse)

Gene Symbol

TIMD2

UniProt

NP_599010

Additional TIM-2 Products

Product Documents for Mouse TIM‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TIM‑2 Antibody

For research use only

Citations for Mouse TIM‑2 Antibody

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Protocols

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