Mouse TNF-alpha Antibody [Biotin]

R&D Systems | Catalog # BAF410

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

Western Blot, ELISA Detection, ELISA Development, ELISA Development (Detection), Luminex Development

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all TNF-alpha Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse TNF-alpha (R&D Systems, Catalog # 410-MT)
Leu80-Leu235
Accession # P06804

Specificity

Detects mouse TNF-alpha in ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse TNF-alpha Antibody [Biotin]

Mouse TNF-alpha ELISA Standard Curve

Mouse TNF-alpha ELISA Standard Curve

Recombinant Mouse TNF‑ alpha aa 80-235 (Catalog # 410-MT) was serially diluted and captured by Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse TNF-alpha Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse TNF‑ alpha (Catalog # 410-MT)

Mouse TNF-alpha Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human/Mouse TNF‑ alpha Antibody (Catalog # AF-410-NA)
  • Standard: Recombinant Mouse TNF-alpha (aa 80-235) Protein (Catalog # 410-MT)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF410 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Long Name

Tumor Necrosis Factor alpha

Alternate Names

Cachetin, DIF, TNF, TNF-A, TNFA, TNFalpha, TNFG1F, TNFSF1A, TNFSF2

Entrez Gene IDs

7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)

Gene Symbol

TNF

UniProt

P06804

Additional TNF-alpha Products

Product Documents for Mouse TNF-alpha Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse TNF-alpha Antibody [Biotin]

For research use only

Citations for Mouse TNF-alpha Antibody [Biotin]

Customer Reviews for Mouse TNF-alpha Antibody [Biotin] (2)

4.5 out of 5
2 Customer Ratings
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Showing  1 - 2 of 2 reviews Showing All
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  • Mouse TNF-alpha Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Mouse
    Verified Customer | Posted 12/01/2017
    Mouse TNF-alpha Antibody [Biotin] BAF410
  • Mouse TNF-alpha Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 11/27/2017
    This antibody was used as the detection antibody in an ELISA.
    Mouse TNF-alpha Antibody [Biotin] BAF410

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