Human/Mouse TNF-alpha Antibody

Catalog # Availability Size / Price Qty
AF-410-NA
AF-410-SP

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Detection of Human TNF‑ alpha  by Western Blot.
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Citations (81)
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Human/Mouse TNF-alpha Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects mouse TNF-alpha  in ELISAs. Detects human and mouse TNF-alpha in Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant rat TNF‑ alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse TNF-alpha
Leu80-Leu235
Accession # P06804
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
3-15 µg/mL
Immersion fixed paraffin-embedded sections of Mouse Spleen and Human Spleen.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Mouse TNF-alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TNF‑ alpha Biotinylated Antibody (Catalog # BAF410)

Standard: Recombinant Mouse TNF-alpha (aa 80-235) Protein (Catalog # 410-MT)

Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 1.5-10 ng/mL in the presence of 0.1 ng/mL Recombinant Mouse TNF‑ alpha and actinomycin D.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human TNF-a antibody by Western Blot. View Larger

Detection of Human TNF‑ alpha by Western Blot. Western blot shows conditioned media from CHO Chinese hamster ovary cell line either mock transfected or transfected with human TNF- alpha. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Mouse TNF-a antibody by Western Blot. View Larger

Detection of Mouse TNF‑ alpha by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). Specific bands were detected for TNF-a at approximately 14 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry TNF-a antibody in RAW 264 by Immunocytochemistry (ICC).7 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

TNF‑ alpha in RAW 264.7 Mouse Cell Line. TNF-a was detected in immersion fixed RAW 264.7 mouse monocyte/ macrophage cell line treated with LPS using Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry TNF-a antibody in Mouse T Cells by Immunocytochemistry (ICC). View Larger

TNF‑ alpha in Mouse T Cells. TNF‑ alpha was detected in immersion fixed activated mouse T Cells using 15 µg/mL Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑410‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry TNF-alpha antibody in Mouse Spleen Tissue by Immunohistochemistry (IHC-P). View Larger

TNF-alpha in Mouse Spleen Tissue. TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cytotoxicity Induced by TNF‑ alpha  and Neutralization by Mouse TNF‑ alpha  Antibody. View Larger

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF‑ alpha Antibody. Recombinant Mouse TNF-a (410-MT) induces cytotoxicity in the the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Cytotoxicity elicited by Recombinant Mouse TNF-a (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Human/Mouse TNF-a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA). The ND50 is typically 1.5-10 ng/mL in the presence of the metabolic inhibitor actinomycin D.

Immunohistochemistry View Larger

Detection of TNF‑ alpha in Human Spleen. TNF‑ alpha was detected in immersion fixed paraffin-embedded sections of Human Spleen using Goat Anti-Human/Mouse TNF‑ alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-410-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western Blot Detection of Mouse TNF-alpha by Western Blot View Larger

Detection of Mouse TNF-alpha by Western Blot WNT-5A induces a proinflammatory transformation in mouse microglia. (A, B) iNOS, COX-2 and TNF alpha were detected by immunoblotting in lysates from mouse primary microglia after WNT-5A stimulation (ctrl, 300 ng/ml, 6 hours). (B’) shows TNF alpha levels in the supernatant of primary microglia under ctrl conditions and upon WNT-5A stimulation (ctrl, 300 ng/ml, 24 hours; n = 4). At least three experiments are summarized in the bar graphs. Data are normalized to ctrl. Error bars give s.e.m. (C) Microglial proliferation was assessed by an MTT assay monitoring mitochondrial activity, which is proportional to cell number [see Additional file 1: Figure S5]. Stimulation with WNT-5A (300 ng/ml, 24 hours) increased MTT, which was blocked by PTX (100 ng/ml, overnight) or the MEK1/2 (10 μM) inhibitor, SL327. (D). Experiments were done in triplicate and data from three independent experiments are shown. *, P < 0.01; ***, P < 0.001: Error bars show s.e.m.. (E) Cell tracker (red)-stained primary microglia were seeded on top of a collagen matrix in 35 mm glass bottom dishes. One day after ctrl or 300 ng/ml WNT-5A stimulation, invasion was observed by confocal microscopy and Z-stacking using a Zeiss LSM710 microscope and subsequent analysis with the Bitplane Imaris software. The size of the collagen cube shown is 1,000 (Z) x 1,400 (Y) x 1,400 (X) μm. Three invasion experiments in the absence and presence of the MEK1/2 inhibitor SL327 were quantified. Data are presented in a bar graph (F). *, P < 0.05; error bars show s.e.m.. (G) cDNA of ctrl stimulated (−) and WNT-5A stimulated (+) primary microglia was analyzed by QPCR for expression of inflammatory genes. At least three independent experiments are summarized. Gene expression is normalized to the housekeeping gene GAPDH and expressed as arbitrary units (2-delta ct). *, P < 0.05; **, P < 0.01. COX-2, cyclooxygenase 2; iNOS, inducible nitric oxide synthase; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; n, number; s.e.m., standard error of the mean. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22647544), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor necrosis factor alpha (TNF-alpha, TNF- alpha, TNFA ), also known as Cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. Mouse TNF-alpha consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70%-77% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa molecular weight soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers present on virtually all cell types. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha.

Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

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Citations for Human/Mouse TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

81 Citations: Showing 1 - 10
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  1. Fanca deficiency is associated with alterations in osteoclastogenesis that are rescued by TNF?
    Authors: Oppezzo, A;Monney, L;Kilian, H;Slimani, L;Maczkowiak-Chartois, F;Rosselli, F;
    Cell & bioscience
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice
    Authors: Y Hwang, HC Kim, EJ Shin
    Journal of Neuroinflammation, 2022-06-11;19(1):142.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Molecular mechanism of the TGF?beta/Smad7 signaling pathway in ulcerative colitis
    Authors: B Bai, H Li, L Han, Y Mei, C Hu, Q Mei, J Xu, X Liu
    Molecular Medicine Reports, 2022-02-09;25(4):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022-01-10;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  5. M1 macrophages impair tight junctions between endothelial cells after spinal cord injury
    Authors: Y Luo, F Yao, X Hu, Y Li, Y Chen, Z Li, Z Zhu, S Yu, D Tian, L Cheng, M Zheng, J Jing
    Brain research bulletin, 2022-01-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Lipopolysaccharide- TLR-4 Axis regulates Osteoclastogenesis independent of RANKL/RANK signaling
    Authors: MS AlQranei, LT Senbanjo, H Aljohani, T Hamza, MA Chellaiah
    BMC immunology, 2021-03-25;22(1):23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Cornus officinalis var. koreana Kitam polyphenol extract decreases pro-inflammatory markers in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages by reducing Akt phosphorylation
    Authors: RS Najjar, NS Akhavan, S Pourafshar, BH Arjmandi, RG Feresin
    Journal of ethnopharmacology, 2020-12-24;0(0):113734.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Minocycline reverses IL-17A/TRAF3IP2-mediated p38 MAPK/NF-kappaB/iNOS/NO-dependent cardiomyocyte contractile depression and death
    Authors: T Yoshida, NA Das, AJ Carpenter, R Izadpanah, SA Kumar, S Gautam, SB Bender, U Siebenlist, B Chandrasek
    Cell. Signal., 2020-06-15;73(0):109690.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Cells
    Applications: Neutralization, Western Blot
  9. Adipocytes promote tumor progression and induce PD-L1 expression via TNF-&alpha/IL-6 signaling
    Authors: Z Li, C Zhang, JX Du, J Zhao, MT Shi, MW Jin, H Liu
    Cancer Cell Int., 2020-05-20;20(0):179.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Endothelial autophagy deficiency induces IL6 - dependent endothelial mesenchymal transition and organ fibrosis
    Authors: Y Takagaki, SM Lee, Z Dongqing, M Kitada, K Kanasaki, D Koya
    Autophagy, 2020-01-22;0(0):1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Paclitaxel-activated astrocytes produce mechanical allodynia in mice by releasing tumor necrosis factor-alpha and stromal-derived cell factor 1
    Authors: X Liu, R Tonello, Y Ling, YJ Gao, T Berta
    J Neuroinflammation, 2019-11-10;16(1):209.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  12. The reciprocal interaction between tumor cells and activated fibroblasts mediated by TNF-alpha/IL-33/ST2L signaling promotes gastric cancer metastasis
    Authors: Q Zhou, X Wu, X Wang, Z Yu, T Pan, Z Li, X Chang, Z Jin, J Li, Z Zhu, B Liu, L Su
    Oncogene, 2019-10-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  13. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  14. Macrophage miR-34a Is a Key Regulator of Cholesterol Efflux and Atherosclerosis
    Authors: Y Xu, Y Xu, Y Zhu, H Sun, C Juguilon, F Li, D Fan, L Yin, Y Zhang
    Mol. Ther., 2019-09-12;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Role of Proinflammatory Cytokines in Feedback Modulation of Circadian Clock Gene Rhythms by Saturated Fatty Acids
    Authors: SM Kim, N Neuendorff, DJ Earnest
    Sci Rep, 2019-06-20;9(1):8909.
    Species: Mouse
    Sample Types: Transduced Whole Cells
    Applications: Neutralization
  16. Aspergillus�fumigatus enhances human NK cell activity by regulating M1 macrophage polarization
    Authors: X Zhang, D He, S Gao, Y Wei, L Wang
    Mol Med Rep, 2019-06-06;20(2):1241-1249.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture
  17. Inhibition of tumour necrosis factor alpha in the R6/2 mouse model of Huntington's disease by etanercept treatment
    Authors: J Pido-Lopez, B Tanudjojo, S Farag, MK Bondulich, R Andre, SJ Tabrizi, GP Bates
    Sci Rep, 2019-05-10;9(1):7202.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. MicroRNA-29b Enhances Osteoclast Survival by Targeting BCL-2-Modifying Factor after Lipopolysaccharide Stimulation
    Authors: OJ Sul, M Rajasekara, HJ Park, JH Suh, HS Choi
    Oxid Med Cell Longev, 2019-04-10;2019(0):6018180.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Neuropathology in intrauterine growth restricted newborn piglets is associated with glial activation and proinflammatory status in the brain
    Authors: JA Wixey, KM Lee, SM Miller, K Goasdoue, PB Colditz, S Tracey Bjo, KK Chand
    J Neuroinflammation, 2019-01-08;16(1):5.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. The Traditional Chinese Medicine MLC901 inhibits inflammation processes after focal cerebral ischemia
    Authors: C Widmann, C Gandin, A Petit-Pait, M Lazdunski, C Heurteaux
    Sci Rep, 2018-12-24;8(1):18062.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice
    Authors: RF Righetti, TM Dos Santos, LDN Camargo, LRCRB Aristótele, S Fukuzaki, FCR de Souza, FPR Santana, MVR de Agrela, MM Cruz, MIC Alonso-Val, IS Genaro, BM Saraiva-Ro, EA Leick, MA Martins, CM Prado, IFLC Tibério
    Front Pharmacol, 2018-10-04;9(0):1021.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. A dual noradrenergic mechanism for the relief of neuropathic allodynia by the antidepressant drugs duloxetine and amitriptyline
    Authors: M Kremer, I Yalcin, Y Goumon, X Wurtz, L Nexon, D Daniel, S Megat, RA Ceredig, C Ernst, G Turecki, V Chavant, JF Theroux, A Lacaud, LE Joganah, V Lelievre, D Massotte, PE Lutz, R Gilsbach, E Salvat, M Barrot
    J. Neurosci., 2018-09-24;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Kaempferol targeting on the fibroblast growth factor receptor 3-ribosomal S6 kinase 2 signaling axis prevents the development of rheumatoid arthritis
    Authors: CJ Lee, SJ Moon, JH Jeong, S Lee, MH Lee, SM Yoo, HS Lee, HC Kang, JY Lee, WS Lee, HJ Lee, EK Kim, JY Jhun, ML Cho, JK Min, YY Cho
    Cell Death Dis, 2018-03-14;9(3):401.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  24. AKT1 distinctively suppresses MyD88-depenedent and TRIF-dependent Toll-like receptor signaling in a kinase activity-independent manner
    Authors: K Zenke, M Muroi, KI Tanamoto
    Cell. Signal., 2017-12-11;43(0):32-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Subversion of NK-cell and TNF? Immune Surveillance Drives Tumor Recurrence
    Authors: T Kottke, L Evgin, KG Shim, D Rommelfang, N Boisgeraul, S Zaidi, RM Diaz, J Thompson, E Ilett, M Coffey, P Selby, H Pandha, K Harrington, A Melcher, R Vile
    Cancer Immunol Res, 2017-10-15;5(11):1029-1045.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  26. Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis
    Authors: SH Lee, JY Kwon, SY Kim, K Jung, ML Cho
    Sci Rep, 2017-08-31;7(1):10133.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  27. Gender difference in NASH susceptibility: Roles of hepatocyte Ikk? and Sult1e1
    Authors: N Matsushita, MT Hassanein, M Martinez-C, R Lazaro, SW French, W Xie, K Lai, M Karin, H Tsukamoto
    PLoS ONE, 2017-08-10;12(8):e0181052.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Anti-inflammatory Effects of the Natural Compounds Cortex Phellodendri and Humulus japonicus on Pelvic Inflammatory Disease in Mice
    Authors: Y Oh, YS Kwon, BD Jung
    Int J Med Sci, 2017-07-18;14(8):729-734.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  29. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity
    Authors: KJ Chung, A Chatzigeor, M Economopou, R Garcia-Mar, VI Alexaki, I Mitroulis, M Nati, J Gebler, T Ziemssen, SE Goelz, J Phieler, JH Lim, KP Karalis, T Papayannop, M Blüher, G Hajishenga, T Chavakis
    Nat. Immunol., 2017-04-17;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  30. Deficiency of PTP1B Attenuates Hypothalamic Inflammation via Activation of the JAK2-STAT3 Pathway in Microglia
    Authors: T Tsunekawa, R Banno, A Mizoguchi, M Sugiyama, T Tominaga, T Onoue, D Hagiwara, Y Ito, S Iwama, M Goto, H Suga, Y Sugimura, H Arima
    EBioMedicine, 2017-01-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  31. Inhibiting the NLRP3 inflammasome with MCC950 promotes non-phlogistic clearance of amyloid-? and cognitive function in APP/PS1 mice
    Authors: C Dempsey
    Brain Behav. Immun, 2016-12-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  32. Chronic blockade of the AT2 receptor with PD123319 impairs insulin signaling in C57BL/6 mice
    Authors: M C Muñoz
    Peptides, 2016-12-12;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  33. Oryza sativa (Rice) Hull Extract Inhibits Lipopolysaccharide-Induced Inflammatory Response in RAW264.7 Macrophages by Suppressing Extracellular Signal-regulated Kinase, c-Jun N-terminal Kinase, and Nuclear Factor-?B Activation
    Authors: Yoonsook Kim
    Pharmacogn Mag, 2016-10-01;12(48):295-301.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  34. Tumor-infiltrating monocytes/macrophages promote tumor invasion and migration by upregulating S100A8 and S100A9 expression in cancer cells
    Authors: SY Lim, AE Yuzhalin, AN Gordon-Wee, RJ Muschel
    Oncogene, 2016-04-18;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  35. FcgammaRIIb inhibits immune complex-induced VEGF-A production and intranodal lymphangiogenesis.
    Authors: Clatworthy M, Harford S, Mathews R, Smith K
    Proc Natl Acad Sci U S A, 2014-12-04;111(50):17971-6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  36. No effect of ablation of surfactant protein-D on acute cerebral infarction in mice.
    Authors: Lambertsen K, Ostergaard K, Clausen B, Hansen S, Stenvang J, Thorsen S, Meldgaard M, Kristensen B, Hansen P, Sorensen G, Finsen B
    J Neuroinflammation, 2014-07-19;11(0):123.
    Species: Mouse
    Sample Types: Plasma
    Applications: Functional Assay
  37. Cytokine conditioning enhances systemic delivery and therapy of an oncolytic virus.
    Authors: Ilett E, Kottke T, Donnelly O, Thompson J, Willmon C, Diaz R, Zaidi S, Coffey M, Selby P, Harrington K, Pandha H, Melcher A, Vile R
    Mol Ther, 2014-06-24;22(10):1851-63.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Glimepiride reduces CD14 expression and cytokine secretion from macrophages.
    Authors: Ingham V, Williams A, Bate C
    J Neuroinflammation, 2014-06-21;11(0):115.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  39. Pathophysiology of lung injury induced by common bile duct ligation in mice.
    Authors: Shikata F, Sakaue T, Nakashiro K, Okazaki M, Kurata M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H
    PLoS ONE, 2014-04-14;9(4):e94550.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  40. Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity.
    Authors: Ramkhelawon B, Hennessy E, Menager M, Ray T, Sheedy F, Hutchison S, Wanschel A, Oldebeken S, Geoffrion M, Spiro W, Miller G, McPherson R, Rayner K, Moore K
    Nat Med, 2014-03-02;20(4):377-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Mice lacking C1q are protected from high fat diet-induced hepatic insulin resistance and impaired glucose homeostasis.
    Authors: Hillian, Antoinet, McMullen, Megan R, Sebastian, Becky M, Roychowdhury, Sanjoy, Kashyap, Sangeeta, Schauer, Philip R, Kirwan, John P, Feldstein, Ariel E, Nagy, Laura E
    J Biol Chem, 2013-06-20;288(31):22565-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  42. Regulator of calcineurin 1 suppresses inflammation during respiratory tract infections.
    Authors: Junkins R, MacNeil A, Wu Z, McCormick C, Lin T
    J Immunol, 2013-04-15;190(10):5178-86.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development (Capture)
  43. Wingless-type mammary tumor virus integration site family, member 5A (Wnt5a) regulates human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein 120 (gp120)-induced expression of pro-inflammatory cytokines via the Ca2+/calmodulin-dependent protein kinase II (CaMKII) and c-Jun N-terminal kinase (JNK) signaling pathways.
    Authors: Li B, Shi Y, Shu J, Gao J, Wu P, Tang S
    J Biol Chem, 2013-03-28;288(19):13610-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  44. Defects in the peripheral taste structure and function in the MRL/lpr mouse model of autoimmune disease.
    Authors: Kim A, Feng P, Ohkuri T, Sauers D, Cohn ZJ, Chai J, Nelson T, Bachmanov AA, Huang L, Wang H
    PLoS ONE, 2012-04-19;7(4):e35588.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  45. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  46. IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells.
    Authors: Fisher DT, Chen Q, Skitzki JJ, Muhitch JB, Zhou L, Appenheimer MM, Vardam TD, Weis EL, Passanese J, Wang WC, Gollnick SO, Dewhirst MW, Rose-John S, Repasky EA, Baumann H, Evans SS
    J. Clin. Invest., 2011-09-19;121(10):3846-59.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  47. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009-10-07;183(9):5799-806.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  48. Attenuation of AD-like neuropathology by harnessing peripheral immune cells: local elevation of IL-10 and MMP-9.
    Authors: Koronyo-Hamaoui M, Ko MK, Koronyo Y, Azoulay D, Seksenyan A, Kunis G, Pham M, Bakhsheshian J, Rogeri P, Black KL, Farkas DL, Schwartz M
    J. Neurochem., 2009-09-24;111(6):1409-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  49. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009-09-14;119(10):2954-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  50. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009-07-17;110(6):1931-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  51. Increased cytokine production in IL-18 receptor alpha-deficient cells is associated with dysregulation of suppressors of cytokine signaling (SOCS).
    Authors: Nold-Petry CA, Nold MF, Nielsen JW, Bustamante A, Zepp JA, Storm KA, Hong JW, Kim SH, Dinarello CA
    J. Biol. Chem., 2009-07-10;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  52. Cooperative phagocytes: resident microglia and bone marrow immigrants remove dead photoreceptors in retinal lesions.
    Authors: Joly S, Francke M, Ulbricht E, Beck S, Seeliger M, Hirrlinger P, Hirrlinger J, Lang KS, Zinkernagel M, Odermatt B, Samardzija M, Reichenbach A, Grimm C, Reme CE
    Am. J. Pathol., 2009-05-12;174(6):2310-23.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  53. ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
    Microbes Infect., 2009-03-13;11(4):524-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  54. Rod-Shaped monocytes patrol the brain vasculature and give rise to perivascular macrophages under the influence of proinflammatory cytokines and angiopoietin-2.
    Authors: Audoy-Remus J, Richard JF, Soulet D, Zhou H, Kubes P, Vallieres L
    J. Neurosci., 2008-10-08;28(41):10187-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  55. Hypoxia enhances lysosomal TNF-alpha degradation in mouse peritoneal macrophages.
    Authors: Lahat N, Rahat MA, Kinarty A, Weiss-Cerem L, Pinchevski S, Bitterman H
    Am. J. Physiol., Cell Physiol., 2008-04-23;295(1):C2-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  56. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
    Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
    Biochem. Pharmacol., 2008-04-18;76(1):79-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  57. CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
    Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
    Immunology, 2008-04-03;125(2):272-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  58. Tumor-specific Th17-polarized cells eradicate large established melanoma.
    Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
    Blood, 2008-03-19;112(2):362-73.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  59. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice.
    Authors: Kim HG, Kim NR, Gim MG, Lee JM, Lee SY, Ko MY, Kim JY, Han SH, Chung DK
    J. Immunol., 2008-02-15;180(4):2553-61.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  60. Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin.
    Authors: Amigo M, Paya M, De Rosa S, Terencio MC
    Br. J. Pharmacol., 2007-07-16;152(3):353-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  61. TNF-alpha and IL-1beta mediate inflammatory hypernociception in mice triggered by B1 but not B2 kinin receptor.
    Authors: Cunha TM, Verri WA, Jr, Fukada SY, Guerrero AT, Santodomingo-Garzon T, Poole S, Parada CA, Ferreira SH, Cunha FQ
    Eur. J. Pharmacol., 2007-07-13;573(1):221-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  62. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006-12-15;177(12):8650-7.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
    Applications: ELISA Development
  63. Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice.
    Authors: Penido C, Conte FP, Chagas MS, Rodrigues CA, Pereira JF, Henriques MG
    Inflamm. Res., 2006-11-01;55(11):457-64.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  64. Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
    Authors: Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjostedt A, Edebro H, Forsman M, Bystrom M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
    Infect. Immun., 2006-09-18;74(12):6730-8.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  65. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4).
    Authors: Ramos CD, Fernandes KS, Canetti C, Teixeira MM, Silva JS, Cunha FQ
    Eur. J. Immunol., 2006-08-01;36(8):2025-34.
    Species: Mouse
    Sample Types: Tissue Secretion
    Applications: ELISA Development
  66. Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
    Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
    J. Immunol., 2006-08-01;177(3):1967-74.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  67. Glatiramer acetate fights against Alzheimer's disease by inducing dendritic-like microglia expressing insulin-like growth factor 1.
    Authors: Butovsky O, Koronyo-Hamaoui M, Kunis G, Ophir E, Landa G, Cohen H, Schwartz M
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-24;103(31):11784-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  68. T lineage differentiation from human embryonic stem cells.
    Authors: Galic Z, Kitchen SG, Kacena A, Subramanian A, Burke B, Cortado R, Zack JA
    Proc. Natl. Acad. Sci. U.S.A., 2006-07-14;103(31):11742-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  69. Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
    Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
    J. Immunol., 2006-07-01;177(1):612-20.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  70. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
    Authors: Murata T, Tsuboi M, Hikita K, Kaneda N
    J. Biol. Chem., 2006-06-13;281(32):22503-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  71. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006-03-10;106(3):418-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  72. Microglia activated by IL-4 or IFN-gamma differentially induce neurogenesis and oligodendrogenesis from adult stem/progenitor cells.
    Authors: Butovsky O, Ziv Y, Schwartz A, Landa G, Talpalar AE, Pluchino S, Martino G, Schwartz M
    Mol. Cell. Neurosci., 2005-11-16;31(1):149-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  73. Tumor necrosis factor-like weak inducer of apoptosis increases the permeability of the neurovascular unit through nuclear factor-kappa B pathway activation.
    Authors: Polavarapu R, Gongora MC, Winkles JA, Yepes M
    J. Neurosci., 2005-11-02;25(44):10094-100.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  74. The sex steroid precursor DHEA accelerates cutaneous wound healing via the estrogen receptors.
    Authors: Mills SJ, Ashworth JJ, Gilliver SC, Hardman MJ, Ashcroft GS
    J. Invest. Dermatol., 2005-11-01;125(5):1053-62.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  75. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005-07-01;302(1):172-81.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development
  76. Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
    Authors: Gonzalez de Aguilar JL, Dupuis L, Larmet Y
    Neurobiol. Dis., 2005-06-01;19(1):129-41.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Neutralization, Western Blot
  77. Selective macrophage suppression during sepsis.
    Authors: Ellaban E, Bolgos G, Remick D
    Cell. Immunol., 2005-02-26;231(1):103-11.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  78. Transcriptional profiling of lipopolysaccharide-induced acute lung injury.
    Authors: Jeyaseelan S, Chu HW, Young SK, Worthen GS
    Infect. Immun., 2004-12-01;72(12):7247-56.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development
  79. Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
    Authors: LaBue M, Colburn KK, Green LM
    Thyroid, 2004-04-01;14(4):249-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation, Western Blot
  80. Role of tumor necrosis factor receptor-1 in the death of retinal ganglion cells following optic nerve crush injury in mice.
    Authors: Tezel G, Yang X, Yang J, Wax MB
    Brain Res., 2004-01-23;996(2):202-12.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  81. Elevated levels of tumor necrosis factor alpha (TNF-alpha) in human immunodeficiency virus type 1-transgenic mice: prevention of death by antibody to TNF-alpha.
    Authors: De SK, Devadas K, Notkins AL
    J. Virol., 2002-11-01;76(22):11710-4.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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Human/Mouse TNF-alpha Antibody
By Tina Yuan on 11/23/2020
Application: IHC Sample Tested: mouse oral tissue Species: Mouse

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By Anonymous on 02/27/2018
Application: B/N Sample Tested: L929 Species: Mouse

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Application: ELISA Sample Tested: Recombinant protein Species: Mouse

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Application: ELISA Sample Tested: Liver tissue Species: Mouse

Mouse TNF-alpha Antibody
By Michelle Chen on 05/10/2017
Application: B/N Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse