Mouse TNF RI/TNFRSF1A Antibody

(10 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human TNF RI is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse TNF RI
    Ile22-Ala212
    Accession # P25118
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Perfusion fixed frozen sections of mouse intestine
  • Agonist Activity
    Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
    The ED50 for this effect is typically 0.05-0.15 μg/mL.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of L‑929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of TNF RI/TNFRSF1A in L‑929 Mouse Cell Line by Flow Cytometry. L‑929 mouse fibroblast cell line was stained with Goat Anti‑Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB, filled histo­gram) or control antibody (Catalog # AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R‑p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF‑ alpha and TNF‑ beta /lymphotoxin. Each subunit contains four TNF‑ alpha trimer‑binding cysteine‑rich domains (CRD) in its extracellular domain (ECD) (1‑6). TNF‑ alpha binding to TNF R1 induces the sequestration of TNFRI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM‑17/TACE (7, 8). Release of the 28‑34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (12). Such release helps to resolve inflammatory reactions as it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF‑ alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1‑3). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence and a 191 aa ECD with a PLAD domain (6). This mediates constitutive trimer formation. The PLAD domain is followed by four CRDs, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (16). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, procine, human and rat TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII.

  • References:
    1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
    2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
    3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
    4. Banner, D.W et al. (1993) Cell 73:431.
    5. Medvedev, A.E. et al. (1996) J. Biol. Chem. 271:9778.
    6. Chan, F.K. et al. (2000) Science 288:2351.
    7. Legler, D.F. et al. (2003) Immunity 18:655.
    8. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
    9. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
    10. Jin, L. et al. (2000) J. Immunol. 165:5153.
    11. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
    12. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
    13. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
    14. McDermott, M.F. et al. (1999) Cell 97:133.
    15. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
    16. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
  • Long Name:
    Tumor Necrosis Factor Receptor I
  • Entrez Gene IDs:
    7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
  • Alternate Names:
    CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
    Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin
    Sci Rep, 2017;7(0):44189.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury
    Authors: S Martens, M Jeong, W Tonnus, F Feldmann, S Hofmans, V Goossens, N Takahashi, JH Bräsen, EW Lee, P Van der Ve, J Joossens, K Augustyns, S Fulda, A Linkermann, J Song, P Vandenabee
    Cell Death Dis, 2017;8(6):e2904.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  3. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
    Authors: Freeman B, Meyer C, Slifka M
    J Virol, 2014;88(13):7474-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Transforming growth factor beta-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling.
    Authors: Li L, Chen Y, Doan J, Murray J, Molkentin J, Liu Q
    Circulation, 2014;130(24):2162-72.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
    Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
    J Immunol, 2014;193(4):2024-33.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012;209(6):1219-34.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009;119(10):2954-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  8. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
    Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
    Nat. Immunol., 2008;9(9):1047-54.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  9. P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
    Authors: Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P
    J. Biol. Chem., 2006;281(41):30684-96.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  10. Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
    Authors: LaBue M, Colburn KK, Green LM
    Thyroid, 2004;14(4):249-62.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
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