Mouse TRAIL R2/TNFRSF10B Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) TRAIL R2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse TRAIL R2/TNFRSF10B
    Asn53-Ser177
    Accession # Q6GSD9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse TRAIL R2/TNFRSF10B
  • Flow Cytometry
    0.25 µg/106 cells
    A20 mouse cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize TRAIL/TNFSF10-induced cytotoxicity in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.4-1.2 µg/mL in the presence of 12 ng/mL Recombinant Human TRAIL/TNFSF10, a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody, and 1 µg/mL actinomycin D.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cytotoxicity Induced by TRAIL/TNFSF10 and Neutralization by Mouse TRAIL R2/TNFRSF10B Antibody. In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRAIL R2/TNFRSF10B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF721). The ND50 is typically 0.4-1.2 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TRAIL R2/TNFRSF10B

Mouse TRAIL R2, also called DR5, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). Mouse TRAIL R2 cDNA encodes a 381 amino acid residue precursor protein containing an extracellular cysteine-rich domain, a transmembrane domain and a cytoplasmic death domain. Human and mouse TRAIL R2 share 49% amino acid sequence similarity. The death domains of human TRAIL R1 and TRAIL R2 share high homology with the death domain of mouse TRAIL R2, 76% and 79%, respectively. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The mouse TRAIL R2/Fc chimera neutralizes the ability of rhTRAIL to induce apoptosis. Besides the death domain containing receptors TRAIL R2 and TRAIL R1/DR4, three TRAIL decoy receptors, TRAIL R3/DcR1, TRAIL R4/DcR2, and OPG, have been reported.

  • References:
    1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
    2. Walczak, H. et al. (1997) EMBO J. 16:5386.
    3. Golstein, P. (1997) Curr. Biol. 7:R750.
    4. Wu, G.S. et al. (1999) Cancer Research 59:2770.
  • Long Name:
    TRAIL Receptor 2
  • Entrez Gene IDs:
    8795 (Human); 21933 (Mouse)
  • Alternate Names:
    CD262 antigen; CD262; death domain containing receptor for TRAIL/Apo-2L; Death receptor 5; DR5; DR5TRICK2B; Fas-like protein; KILLER/DR5; KILLERapoptosis inducing protein TRICK2A/2B; TNF-related apoptosis-inducing ligand receptor 2; TNFRSF10B; TRAIL R2; TRAIL receptor 2; TRAILR2; TRAIL-R2apoptosis inducing receptor TRAIL-R2; TRAILR2cytotoxic TRAIL receptor-2; TRICK2; TRICK2A; TRICKB; tumor necrosis factor receptor superfamily member 10B; tumor necrosis factor receptor superfamily, member 10b; tumor necrosis factor receptor-like protein ZTNFR9; ZTNFR9
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. IAP antagonists sensitize murine osteosarcoma cells to killing by TNF?
    Oncotarget, 2016;7(23):33866-86.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009;113(24):6120-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. P-glycoprotein enhances TRAIL-triggered apoptosis in multidrug resistant cancer cells by interacting with the death receptor DR5.
    Authors: Park SJ, Wu CH, Choi MR, Najafi F, Emami A, Safa AR
    Biochem. Pharmacol., 2006;72(3):293-307.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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