Detects porcine GM‑CSF in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant rat GM‑CSF is observed, 15% cross-reactivity with recombinant human GM‑CSF is observed, and 5% cross-reactivity with recombinant mouse GM‑CSF is observed.
Polyclonal Goat IgG
E. coli-derived recombinant porcine GM-CSF Ala18-Lys144 Accession # Q29118
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.2-1.0 µg/mL in the presence of 10 ng/mL Recombinant Porcine GM‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by GM‑CSF and Neutralization by Porcine GM‑CSF Antibody. Recombinant Porcine GM‑CSF (Catalog # 711-PG) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine GM‑CSF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Porcine GM‑CSF Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF711). The ND50 is typically 0.2-1.0 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.
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