Rat beta -1,3-Glucuronyltransferase 1/B3GAT1 Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in ELISA.
  • Source
    Monoclonal Mouse IgG1 Clone # 882302
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat beta ‑1,3‑Glucuronyltransferase 1/B3GAT1
    Asp75-Ile347
    Accession # NP_445455
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
beta ‑1,3-Glucuronyltransferase 1/B3GAT1 in Rat Cortical Stem Cells.

beta ‑1,3-Glucuronyltransferase 1/B3GAT1 was detected in immersion fixed differentiated rat cortical stem cells using Mouse Anti-Rat beta ‑1,3-Glucuronyltransferase 1/B3GAT1 Monoclonal Antibody (Catalog # MAB6698) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). Oligo2 was also detected in the cells using Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Cells were counterstained with DAPI (blue). Specific staining of B3GAT1 was localized to transmembrane Golgi. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: beta-1,3-Glucuronyltransferase 1/B3GAT1

B3GAT1 is a key enzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 1-4GlcNAc-) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK-1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc- (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3).The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell‑cell and cell‑substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single‑pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

  • References:
    1. Terayama, K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6093.
    2. Shogo, O. et al. (1992) J. Biol. Chem. 267: 22711.
    3. Kakuda, S. et al. (2005) Glycobiology 2:203.
    4. Bollensen, E. and Schachner, M. (1987) Neurosci Lett. 82:77.
    5. Wu, Z.L. et al. (2011) Glycobiology 21:727.
  • Long Name:
    UDP-GlcUA:glycoprotein beta-1,3-Glucuronyltransferase
  • Entrez Gene IDs:
    27087 (Human); 76898 (Mouse); 117108 (Rat)
  • Alternate Names:
    B3GAT1; beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Beta-1,3-glucuronyltransferase 1; CD57; EC 2.4.1.135; galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GLCATP; GlcAT-P; GLCATPGLCUATP; GLCUATP; glcUAT-P; Glucuronosyltransferase P; HNK1; LEU7; NK1; NK-1; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
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