Rat CCR2 Antibody Summary
Accession # O55193
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) rat CCR2 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CCR2 Monoclonal Antibody (Catalog # MAB8368R) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2b isotype control antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Rat CCR2 shares 95% aa sequence identity with mouse CCR2.
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