Key Product Details

Species Reactivity

Rat

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 868013
View all CXCR3 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with rat CXCR3
Accession # Q9J119

Specificity

Detects rat CXCR3 in ELISA. Stains rat CXCR3 transfected cells but not irrelevant transfectants by Flow Cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Rat CXCR3 Antibody

Detection of CXCR3 antibody in CHO Chinese Hamster Cell Line Transfected with Rat CXCR3 and eGFP antibody by Flow Cytometry.

Detection of CXCR3 in CHO Chinese Hamster Cell Line Transfected with Rat CXCR3 and eGFP by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with rat CXCR3 and eGFP was stained with either (A) Mouse Anti-Rat CXCR3 Monoclonal Antibody (Catalog # MAB8109) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Rat CXCR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: CHO Chinese hamster ovary cell line transfected with rat CXCR3 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR3

CXCR3, also known as CD183, is an approximately 41 kDa (predicted) 7-transmembrane spanning receptor for the chemokines CXCL9, CXCL10, and CXCL11. It is expressed on activated T cells, B cells, and NK cells during inflammation. It is additionally upregulated on solid tumor cells, tumor endothelium, and cancer stem cells. CXCR3 plays a role in leukocyte recruitment to sites of inflammation. It also contributes to Th1 biased responses during autoimune diseases rheumatoid arthritis, systemic erythematosis, and type 1 diabetes. Rat CXCR3 shares 86% and 96% amino acid sequence identity with human and mouse CXCR3, respectively.

Alternate Names

CD183, CXCR3, GPR9

Entrez Gene IDs

2833 (Human); 12766 (Mouse); 84475 (Rat)

Gene Symbol

CXCR3

UniProt

Q9J119

Additional CXCR3 Products

Product Documents for Rat CXCR3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CXCR3 Antibody

For research use only

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