Rat EphB1 Antibody

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EphB1 in Rat Brain.
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Product Details
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Rat EphB1 Antibody Summary

Species Reactivity
Detects rat EphB1 in direct ELISAs.
Monoclonal Mouse IgG2A Clone # 917118
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant rat EphB1
Accession # P09759
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry EphB1 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

EphB1 in Rat Brain. EphB1 was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglia) using Mouse Anti-Rat EphB1 Monoclonal Antibody (Catalog # MAB5422) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in small-sized neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB1

EphB1, also known as Elk, Cek6, Net, and Hek6, is a type I transmembrane receptor tyrosine kinase with two extracellular fibronectin type III domains. EphB1 binds Ephrin B1, B2, and B3 and plays an important role in nervous system development and axonal migration. Within the extracellular domain, rat EphB1 shares 99% amino acid sequence identity with human and mouse EphB1.

Long Name
Eph Receptor B1
Entrez Gene IDs
2047 (Human); 270190 (Mouse); 24338 (Rat)
Alternate Names
Cek6; EC 2.7.10; EC; EK6; Elk; EPH receptor B1; eph tyrosine kinase 2; EphB1; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2FLJ37986; Hek6; Net; NETHek6; soluble EPHB1 variant 1; Tyrosine-protein kinase receptor EPH-2

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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