Rat Fas/TNFRSF6/CD95 Antibody

Catalog # Availability Size / Price Qty
AF2159
AF2159-SP
Fas/TNFRSF6/CD95 in Rat Liver.
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Product Details
Citations (2)
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Rat Fas/TNFRSF6/CD95 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Fas/TNFRSF6/CD95 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Fas, recombinant mouse Fas, and recombinant feline Fas is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Fas/TNFRSF6/CD95
Gln22-Lys170
Accession # NP_631933
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 2159-FA)
Flow Cytometry
2.5 µg/106 cells
Rat splenocytes
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Fas/TNFRSF6/CD95 in Rat Liver. View Larger

Fas/TNFRSF6/CD95 in Rat Liver. Fas/TNFRSF6/CD95 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2159) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cell membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, belongs to the death receptor family, which is a subfamily of the TNF receptor superfamily (1). Death receptors contain a cytoplasmic death domain (DD), which is required for transducing apoptotic signals. Engagement of Fas by its ligand (FasL) or agonistic anti-Fas antibodies induces dimerization and oligomerization of preformed Fas trimers. The activated receptor recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC) that also contains caspases. Upon activation, the caspases initiate a signaling cascade that induces the characteristic apoptotic phenotypes (2). Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. FasL is a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites (3).

Fas plays a role in the down-regulation of the immune reaction and has been shown to be an essential mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B-lymphocytes. Both human and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (4). Besides the perforin/granzyme-based mechanism, the Fas-FasL system has been identified as the alternate pathway for CTL-mediated cytotoxicity (5). FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells (6). Rat Fas cDNA encodes a 324 amino acid residue type 1 membrane protein. The extracellular domain of rat Fas shares 54.1% and 66.7% amino acid sequence identity with that of human and mouse Fas, respectively.

References
  1. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.
  2. Thorburn, S. (2003) Cellular Signaling 16:139.
  3. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.
  4. Siegel, R.M. et al. (2003) Immunol. Res. 27:499.
  5. Barry, M. et al. (2000) Mol. Cell Biol. 20:3781.
  6. Barreiro, R. et al. (2004) J. Immunol. 173:1519.
Long Name
Fibroblast-associated
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Product Datasheets

Citations for Rat Fas/TNFRSF6/CD95 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. IL-12, IL-15, and IL-18 pre-activated NK cells target resistant T cell acute lymphoblastic leukemia and delay leukemia development in vivo
    Authors: M Boieri, A Ulvmoen, A Sudworth, C Lendrem, M Collin, AM Dickinson, L Kveberg, M Inngjerdin
    Oncoimmunology, 2017;6(3):e1274478.
  2. Kruppel-like factor 6 rendered rat Schwann cell more sensitive to apoptosis via upregulating FAS expression.
    Authors: Gui T, Wang Y, Zhang L, Wang W, Zhu H, Ding W
    PLoS ONE, 2013;8(12):e82449.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB

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