|Fas/TNFRSF6/CD95 in Rat Liver. Fas/TNFRSF6/CD95 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2159) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to hepatocyte cell membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.|
Fas, also known as APO-1, CD95, and TNFRSF6, belongs to the death receptor family, which is a subfamily of the TNF receptor superfamily (1). Death receptors contain a cytoplasmic death domain (DD), which is required for transducing apoptotic signals. Engagement of Fas by its ligand (FasL) or agonistic anti-Fas antibodies induces dimerization and oligomerization of preformed Fas trimers. The activated receptor recruits the adaptor molecule FADD to form the Death-Inducing Signaling Complex (DISC) that also contains caspases. Upon activation, the caspases initiate a signaling cascade that induces the characteristic apoptotic phenotypes (2). Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. FasL is a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T-lymphocytes, NK cells, and in tissues with immune-privileged sites (3).
Fas plays a role in the down-regulation of the immune reaction and has been shown to be an essential mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B-lymphocytes. Both human and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity (4). Besides the perforin/granzyme-based mechanism, the Fas-FasL system has been identified as the alternate pathway for CTL-mediated cytotoxicity (5). FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells (6). Rat Fas cDNA encodes a 324 amino acid residue type 1 membrane protein. The extracellular domain of rat Fas shares 54.1% and 66.7% amino acid sequence identity with that of human and mouse Fas, respectively.
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Showing Results 1 - 1 of 1
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided