Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse, Rat, Porcine, Lizard - Gecko

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunofluorescence, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Growth Hormone Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244

Specificity

Detects rat Growth Hormone in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rat Growth Hormone Antibody

Growth Hormone antibody in Rat Pituitary by Immunohistochemistry (IHC-Fr).

Growth Hormone in Rat Pituitary.

Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat Growth Hormone Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of rat embryo (15 d.p.c.) and perfusion fixed frozen sections of rat pituitary

Western Blot

0.1 µg/mL
Sample: Rat Growth Hormone

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Growth Hormone

Growth Hormone, also named somatotropin, belongs to the somatotropin/prolactin superfamily. It is synthesized in the anterior pituitary gland. Growth Hormone regulates body growth by stimulating the secretion of IGF-I by liver and other tissues.

Alternate Names

GH-N, GH1, GHB5, GHN, IGHD1A, IGHD2, Somatotropin

Entrez Gene IDs

2688 (Human)

Gene Symbol

GH1

UniProt

P01244

Additional Growth Hormone Products

Product Documents for Rat Growth Hormone Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat Growth Hormone Antibody

For research use only

Citations for Rat Growth Hormone Antibody

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Protocols

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