Rat ICAM-1/CD54 Antibody

Catalog # Availability Size / Price Qty
AF583
AF583-SP
Detection of Rat ICAM‑1/CD54 by Western Blot.
2 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Rat ICAM-1/CD54 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM‑3, and rmICAM‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
Gln28-Thr493
Accession # Q00238
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Adhesion Blockade
The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Rat ICAM-1 Fc Chimera (Catalog # 583-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Rat ICAM‑1/CD54 by Western Blot. View Larger

Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry ICAM‑1/CD54 in Rat Brain. View Larger

ICAM‑1/CD54 in Rat Brain. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References
  1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
  2. Siu, G. et al. (1989) J. Immunol. 143:3813.
  3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

Product Datasheets

Citations for Rat ICAM-1/CD54 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs
    Authors: G Hu, J Wang, D Hong, T Zhang, H Duan, X Mu, Z Yang
    BMC Complement Altern Med, 2017;17(1):38.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  2. Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis
    Authors: J Huang, S Han, Q Sun, Y Zhao, J Liu, X Yuan, W Mao, B Peng, W Liu, J Yin, X He
    Cell Biosci, 2017;7(0):31.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Sexual dimorphism in the hepatic protein response to a moderate trans fat diet in senescence-accelerated mice
    Authors: SA Bloomer, KE Wellen, GC Henderson
    Lipids Health Dis, 2017;16(1):243.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  4. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  5. Sustained elevation of NF-?B activity sensitizes offspring of maternal inflammation to hypertension via impairing PGC-1? recovery
    Sci Rep, 2016;6(0):32642.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  6. Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
    Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
    PLoS ONE, 2016;11(1):e0146438.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: WB
  7. Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
    Authors: Hanusch C, Nowak K, Gill IS, Torlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
    Clin. Exp. Immunol., 2007;149(2):364-71.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  8. Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
    Authors: Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
    Diabetes, 2005;54(12):3418-26.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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Rat ICAM-1/CD54 Antibody
By Anonymous on 09/06/2016
Application: IHC Sample Tested: Brain tissue Species: Rat