Rat ICAM-1/CD54 Antibody

(7 citations)
(1 Review)
  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat ICAM-1/CD54 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse (rm) ICAM-1 and 5% cross-reactivity with recombinant human (rh) ICAM-1, rhICAM‑3, and rmICAM‑2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat ICAM-1/CD54
    Gln28-Thr493
    Accession # Q00238
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Adhesion Blockade
    The adhesion of HSB2 human peripheral blood acute lymphoblastic leukemia cells (5 x 104 cells/well) to immobilized Recombinant Rat ICAM-1 Fc Chimera (Catalog # 583-IC, 12.5 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat ICAM‑1/CD54 by Western Blot. Western blot shows lysates of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ICAM‑1/CD54 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
ICAM‑1/CD54 in Rat Brain. ICAM‑1/CD54 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF583) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and lipolysaccharide (LPS). Rat and human ICAM-1 share approximately 52% amino acid identity. Rat and mouse ICAM-1 share approximately 52% amino acid identity.

Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

  • References:
    1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
    2. Siu, G. et al. (1989) J. Immunol. 143:3813.
    3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.
  • Long Name:
    Intercellular Adhesion Molecule 1
  • Entrez Gene IDs:
    3383 (Human); 15894 (Mouse); 25464 (Rat)
  • Alternate Names:
    BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Kv1.3 channel blocker (ImKTx88) maintains blood-brain barrier in experimental autoimmune encephalomyelitis
    Authors: J Huang, S Han, Q Sun, Y Zhao, J Liu, X Yuan, W Mao, B Peng, W Liu, J Yin, X He
    Cell Biosci, 2017;7(0):31.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  2. Effects of aqueous extracts of Taraxacum Officinale on expression of tumor necrosis factor-alpha and intracellular adhesion molecule 1 in LPS-stimulated RMMVECs
    Authors: G Hu, J Wang, D Hong, T Zhang, H Duan, X Mu, Z Yang
    BMC Complement Altern Med, 2017;17(1):38.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  3. Astaxanthin Inhibits Expression of Retinal Oxidative Stress and Inflammatory Mediators in Streptozotocin-Induced Diabetic Rats.
    Authors: Yeh P, Huang H, Yang C, Yang W, Yang C
    PLoS ONE, 2016;11(1):e0146438.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  4. Sustained elevation of NF-?B activity sensitizes offspring of maternal inflammation to hypertension via impairing PGC-1? recovery
    Sci Rep, 2016;6(0):32642.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  5. Disrupting the blood-brain barrier by focused ultrasound induces sterile inflammation
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  6. Hypothermic preservation of lung allograft inhibits cytokine-induced chemoattractant-1, endothelial leucocyte adhesion molecule, vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression.
    Authors: Hanusch C, Nowak K, Gill IS, Torlitz P, Rafat N, Mueller AM, Van Ackern KC, Yard B, Beck GC
    Clin. Exp. Immunol., 2007;149(2):364-71.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
  7. Aspirin at low-intermediate concentrations protects retinal vessels in experimental diabetic retinopathy through non-platelet-mediated effects.
    Authors: Sun W, Gerhardinger C, Dagher Z, Hoehn T, Lorenzi M
    Diabetes, 2005;54(12):3418-26.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

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Goat IgG (H+L) APC-conjugated Antibody

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Goat IgG Horseradish Peroxidase-conjugated Antibody

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Donkey Anti-Goat IgG Antibody

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Donkey Anti-Goat IgG Biotinylated Antibody

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Goat IgG VisUCyte HRP Polymer

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Goat IgG (H+L) Fluorescein-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

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Chicken Anti-Goat IgG Biotinylated Antibody

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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 5 (Based on 1 review)

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Immunohistochemistry Rat ICAM-1/CD54 Antibody AF583
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 IHC Anonymous 09/06/2016
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Immunohistochemistry Rat ICAM-1/CD54 Antibody AF583
Immunohistochemistry: Rat ICAM-1/CD54 Antibody [AF583]

Summary

ApplicationImmunohistochemistry
Sample TestedBrain tissue

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