Rat IL-1 alpha /IL-1F1 Antibody

Catalog # Availability Size / Price Qty
MAB500
MAB500-SP
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (1)

Rat IL-1 alpha /IL-1F1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IL‑1 alpha /IL-1F1 in ELISAs and Western blots. In Western blots, does not cross-react with recombinant human IL‑1 alpha, recombinant mouse IL‑1 alpha, recombinant porcine IL‑1 alpha, rhIL‑1 beta, or recombinant rat IL‑1 beta.
Source
Monoclonal Mouse IgG1 Clone # 59015
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant rat IL‑1 alpha /IL-1F1
Ser115-Ser270
Accession # P16598
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat IL‑1 alpha /IL‑1F1 (Catalog # 500-RL)

Rat IL-1 alpha /IL-1F1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat IL‑1 alpha /IL‑1F1 Biotinylated Antibody (Catalog # BAF500)

Standard: Recombinant Rat IL-1 alpha/IL-1F1 Protein (Catalog # 500-RL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid (aa) sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, evidence suggests that IL-1 is also temporally upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the aa sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

References
  1. Dower, S.K. and J.Z. Sims (1994) Guidebook to Cytokines and Their Receptors, Nicola, N.A., ed., Oxford University Press, New York p. 17.
Long Name
Interleukin 1 alpha
Entrez Gene IDs
3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
Alternate Names
BAF; Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; LAF; LEM; preinterleukin 1 alpha; pro-interleukin-1-alpha

Product Datasheets

Citation for Rat IL-1 alpha /IL-1F1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cytokine production by mouse myeloid dendritic cells in relation to differentiation and terminal maturation induced by lipopolysaccharide or CD40 ligation.
    Authors: Morelli AE, Zahorchak AF, Larregina AT
    Blood, 2001;98(5):1512-23.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Rat IL-1 alpha /IL-1F1 Antibody
By Anonymous on 11/07/2017
Application: ELISA Sample Tested: Serum Species: Rat

Together with BAF500 a sandwich ELISA was built to measure rat IL-1a in serum samples.