Rat IL‑1 beta /IL‑1F2 Antibody

Name: Rat IL‑1 beta /IL‑1F2 Antibody
Brand: R&D Systems
SKU: MAB5011
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # MAB5011

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Citations (10)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 38123

View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat IL-1 beta
Val117-Ser268
Accession # Q63264

Specificity

Detects rat IL-1 beta in direct ELISAs and Western blots. In these formats, this antibody shows approximately 100% cross‑reactivity with recombinant mouse (rm) IL-1 beta and rcrIL-1 beta and no cross-reactivity with rfIL‑1 beta, rhIL-1 beta, or rrmIL-1 beta.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Rat IL‑1 beta /IL‑1F2 Antibody

Detection of Rat IL-1 beta /IL-1F2 by Western Blot

Plantaris protein content results for interleukin-1 beta (IL-1 beta ) (A). Data were normalized to CON. Values are mean ± SEM (CON: n = 4; SNL: n = 4; 100CUR: n = 4; 50CMO: n = 4). Φ p < 0.050 vs. ipsilateral leg. The representative blot images display IL-1 beta and alpha -tubulin for CON, SNL, 100CUR, and 50CMO groups (B). MW, molecular weight (kDa). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1467-3045/46/11/742), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Rat IL‑1 beta /IL‑1F2 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat IL‑1 beta /IL‑1F2 (Catalog # 501-RL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.