Rat L-Selectin/CD62L Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat L‑Selectin/CD62L in direct ELISAs and Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse L‑Selectin and 5% cross-reactivity with recombinant human L‑Selectin is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat L‑Selectin/CD62L
    Trp39-Asn332
    Accession # P30836
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Rat L-Selectin/CD62L Fc Chimera (Catalog # 1534-LS)
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
L‑Selectin/CD62L in Rat Thymus. L‑Selectin/CD62L was detected in perfusion fixed frozen sections of rat thymus using 15 µg/mL Goat Anti-Rat L‑Selectin/CD62L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1534) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: L-Selectin/CD62L

L-Selectin (also known as Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is a member of the Selectin family of cell surface molecules which include E-Selectin and P-Selectin. All Selectins have an extracellular domain composed of an amino-terminal calcium-dependent lectin domain, an epidermal growth factor (EGF)-like domain, two to nine short consensus repeat (SCR) units, a transmembrane domain, and a cytoplasmic tail. L-Selectin expression is limited to hematopoietic cells, with most leukocytes expressing L-Selectin at some stage of differentiation. The majority of myeloid cells, B cells, and virgin T cells express L-Selectin, while only a sub-population of memory T cells and NK cells express L-Selectin. Lymphocytes and neutrophils exhibit a reversible loss of L-Selectin after cellular activation that results from endoproteolytic release of the extracellular portion of receptor from the cell surface. Cleavage of L-Selectin from the cell surface results in a high circulating level of functionally active soluble L-Selectin. All selectins bind sialytated and fucosylated oligosaccharides that are linked to glycoproteins and glycolipids. L-Selectin specifically binds to at least three different heavily glycosolylated mucin-like proteins: GlyCAM-1, CD34, and MAdCAM-1. Multiple studies indicated that L-Selectin, P-Selectin E-Selectin collaborate to mediate the initial binding of leukocytes to endothelium at sites of tissue injury and inflammation, producing the characteristic “rolling” of leukocytes along the endothelium. L-Selectin knockout mice have a 70% decrease in rolling leukocytes in exposed mesentery and have impaired neutrophil and monocyte migration into areas of inflammation. Additionally, L-Selectin knockout mice have relatively few lymphocytes present in peripheral lymph nodes and Peyer’s patches. Short-term in vivo homing experiments in L-Selectin deficient mice demonstrate that L-Selectin is involved in lymphocyte homing to Peyer’s patches and mesenteric lymph nodes in the gut. Rat and human L-Selectin share 77% amino acid sequence homology. Rat and mouse L-Selection share 83% amino acid sequence homology (1, 2).

  • References:
    1. Tedder, T.F. et al. (1995) FASEB Journ. 9:866.
    2. McEver, R.P. et al. (1995) J. Biol. Chem. 270:11025.
  • Entrez Gene IDs:
    6402 (Human); 20343 (Mouse); 29259 (Rat)
  • Alternate Names:
    CD62L antigen; CD62L; gp90-MEL; hLHRc; LAM1; LAM-1; LAM1LECAM1; LECAM1; LEU8; Leu-8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; LNHR; LNHRTQ1; LSEL; L-Selectin; LYAM1; Lyam-1; LYAM1CD62 antigen-like family member L; Lymph node homing receptor; lymphocyte adhesion molecule 1; pln homing receptor; PLNHR; selectin L; SELL; TQ1
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