Rat MAG/Siglec-4a Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat MAG/Siglec‑4a in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG2A Clone # 118423
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant rat MAG/Siglec‑4a
    Gly20-Pro516
    Accession # P07722
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat MAG/Siglec‑4a Fc Chimera (Catalog # 538-MG)
  • Neutralization
    Measured by its ability to neutralize MAG/Siglec-4a-mediated adhesion of human red blood cells. Kelm, S. et al. (1994) Current Biology 4:965. At 40 μg/mL, Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a Fc Chimera-dependent adhesion of human red blood cells.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Adhesion Mediated by MAG/Siglec-4a and Neutralization by Rat MAG/Sigle-4a Antibody. Recombinant Rat MAG/Siglec-4a Fc Chimera (Catalog # 538-MG), immobilized onto a microplate, supports the adhesion of human red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat MAG/Siglec-4a Fc Chimera (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat MAG/Siglec-4a Monoclonal Antibody (Catalog # MAB538). At 40 µg/mL, this antibody will neutralize approximately 60-85% of Recombinant Rat MAG/Siglec-4a-dependent adhesion of human red blood cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MAG/Siglec-4a

MAG (Myelin-Associated Glycoprotein), a type I transmembrane glycoprotein containing five Ig-like domains in its extracellular domain is an adhesion molecule belonging to the immunoglobin superfamily. Within this superfamily, MAG, CD22, CD33, Schwann cell myelin protein, and sialoadhesin which bind specifically to cell‑surface glycan containing sialic acid residues define the I-type sialyl lectin subgroup, also called the sialoadhesin family. Sialoadhesins mediate diverse biological processes through recognition of specific sialyted glycans on cell surface. MAG is expressed on myelinating oligodenrocytes and Schwann cells, and preferentially recognizes alpha 2, 3-linked sialic acid on O-linked glycans and gangliosides. MAG exists as two isoforms which differ in the sequence and length of the cytoplasmic tail. The large form (71 kDa) and small form (67 kDa) arise from alternative spliced mRNAs. Although MAG might encounter haematopoietic cells and lymphocytes under pathologic conditions, it would normally be expected to interact with neuronal cells. It has been shown that MAG promotes axonal growth from neonatal DRG neurons and embryonic spinal neurons, but is a potent inhibitor of axonal re-growth from adult DRG and postnatal cerebellar neurons. MAG plays an important role in the interaction between axons and myelin. A soluble form of MAG containing the extracellular domain is released from myelin in large quantities and identified in normal human tissues and in tissues from patients with neurological disorders. This soluble MAG might contribute to the lack of CNS neuron regeneration after injury.

  • References:
    1. Kelm, S. et al. (1994) Current Biology 4:965.
    2. McKerracher, L. et al. (1994) Neuron 13:805.
    3. Tang, S. et al. (1997) Molecular and Cellular Neuroscience 9:333.
    4. Cai, D. et al. (1999) Neuron 22:89.
  • Long Name:
    Myelin-associated Glycoprotein
  • Entrez Gene IDs:
    4099 (Human); 17136 (Mouse); 29409 (Rat)
  • Alternate Names:
    GMAsialic acid binding Ig-like lectin 4A; MAG; myelin associated glycoprotein; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; Siglec4a; Siglec-4a; S-MAG
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