Rat Nogo-A aa 2-172 Antibody

Catalog # Availability Size / Price Qty
AF3098
AF3098-SP

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Detection of Rat Nogo-A by Western Blot
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Product Details
Citations (5)
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Rat Nogo-A aa 2-172 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Nogo-A in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat Nogo-A
Glu2-Val172
Accession # Q9JK11
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat Nogo-A Fc Chimera (Catalog # 2445-NG)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of rat brain (caudate putamen)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat Nogo-A by Western Blot View Larger

Detection of Rat Nogo-A by Western Blot RT-PCR analysis and western blot analysis of Nogo-A mRNA and protein production in the hippocampus after TBI. (A) The PCR products from Nogo-A transcription after TBI with the yield of actin as an internal control. The sampling times after TBI are shown on the top (n = 4 in each group). (B) Quantification of Nogo-A mRNA expression by semiquantitative densitometry in conjunction with AlphaEase software (Alpha Innotech Corp.). (C) Time course of Nogo-A protein production after TBI, internal control: beta -actin. Times shown on top represent hours after injury. (D) Quantification of Nogo-A protein by semiquantitative densitometry in conjunction with AlphaEase software (Alpha Innotech Corp.). The data are presented as ratios related to the sham group. Bars represent the means ± SEM values. *p < 0.05 is considered significantly different from sham values using the Mann–Whitney U test. SEM, standard error of the mean; TBI, traumatic brain injury. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat Nogo-A by Western Blot View Larger

Detection of Rat Nogo-A by Western Blot Effects of Nogo-A irrelevant control and antisense oligodeoxynucleotides on hippocampal Nogo-A expression after TBI. (A) RT-PCR analysis of Nogo-A mRNA transcription level. Actin transcription was used as an internal control. (B) The expression of Nogo-A was quantified by densitometry and compared with the data from rats injected with saline (sham), which was normalized to 100%. (C) Western blot analysis of Nogo-A protein level; beta -actin was used as an internal control. (D) Quantification of Nogo-A protein by semiquantitative densitometry in conjunction with AlphaEase software (Alpha Innotech Corp.). The data are presented compared with the sham group. The data are represented as the means ± SEM values (n = 6). *p < 0.05 was considered significantly different from the sham value using the Mann-Whiney U test, and #p < 0.05 was considered significantly different from the TBI with sense values using the Mann-Whiney U test. SEM, standard error of the mean; TBI, traumatic brain injury. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Rat Nogo-A by Western Blot View Larger

Detection of Rat Nogo-A by Western Blot Effects of indomethacin administration on Nogo-A expression. Animals were in one of four groups: sham (no TBI), TBI treatment (TBI eight hours), TBI combined with vehicle administration (TBI + vehicle), and TBI combined with indomethacin administration (TBI + indomethacin). (A) RT-PCR analysis of the expression of Nogo-A among different groups along with the analysis of beta -actin transcription as an internal control. (B) Quantification of Nogo-A expression. (C) Western blot analysis of the expression of Nogo-A among different groups along with the analysis of beta -actin as an internal control. (D) Quantification of Nogo-A expression. Bars represent means ± SEM values (n = 5). *P <0.05 is considered significantly different from the sham value using the Mann–Whitney U test and #P <0.05 is considered significantly different from the TBI value using the Mann–Whitney U test. SEM, standard error of the mean; TBI, traumatic brain injury. Image collected and cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-121), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo-A

Nogo-A, also known as Reticulon-4, is the longest of four splice variants of Nogo. It is a CNS myelin-associated neurite outgrowth inhibitor that is highly expressed in oligodendrocytes. Nogo-A is synthesized as an 1163 amino acid protein and lacks a signal peptide. Within conserved C-terminal reticulon homology domain (RHD), two transmembrane domains, which are separated by a 66 amino acid extracellular loop, exist. Both the N-terminal domain and the 66 amino acid domain (Nogo-66) can be detected on the cell surface and show neurite outgrowth inhibitory activity. The amino acid sequence of rat Nogo-A N-terminal domain is 76% identical to that of human Nogo-A. Within the RHD domain, rat Nogo-A shares 99% and 97% amino acid sequence identity with mouse and human Nogo, respectively.

Long Name
Reticulon 4A
Entrez Gene IDs
57142 (Human); 68585 (Mouse); 83765 (Rat)
Alternate Names
ASY;Nbla00271;Nbla10545;NI220/250;Nogo;NSP;NSP-CL;r;Reticulon-4;Rtn4;RTN4;RTN4-A;RTN4-B1;RTN4-B2;RTN4-C;RTN-X; NI220; NogoA; Nogo-A; RTN4; RTN4A

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Citations for Rat Nogo-A aa 2-172 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Plasticity-Enhancing Effects of Levodopa Treatment after Stroke
    Authors: Daniela Talhada, Niklas Marklund, Tadeusz Wieloch, Enida Kuric, Karsten Ruscher
    International Journal of Molecular Sciences
  2. A proteolytic C-terminal fragment of Nogo-A (reticulon-4A) is released in exosomes and potently inhibits axon regeneration
    Authors: Y Sekine, JA Lindborg, SM Strittmatt
    Nat. Med., 2019-11-20;295(8):2175-2183.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Indomethacin protects rats from neuronal damage induced by traumatic brain injury and suppresses hippocampal IL-1beta release through the inhibition of Nogo-A expression.
    J Neuroinflammation, 2012-06-07;9(0):121.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Oligodendrocyte-myelin glycoprotein and Nogo negatively regulate activity-dependent synaptic plasticity.
    Authors: Raiker SJ, Lee H, Baldwin KT
    J. Neurosci., 2010-09-15;30(37):12432-45.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Molecular basis of the interactions of the Nogo-66 receptor and its homolog NgR2 with myelin-associated glycoprotein: development of NgROMNI-Fc, a novel antagonist of CNS myelin inhibition.
    Authors: Robak LA, Venkatesh K, Lee H, Raiker SJ, Duan Y, Lee-Osbourne J, Hofer T, Mage RG, Rader C, Giger RJ
    J. Neurosci., 2009-05-06;29(18):5768-83.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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