Rat Nogo-A Antibody

  
  • Species Reactivity
    Rat
  • Specificity
    Detects rat Nogo-A in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 387521
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH-coupled rat Nogo-A synthetic peptide
    SYDSIKLEPENPPPYEEA (aa 623-640)
    Accession # Q9JK11
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Nogo‑A in Rat Embryo. Nogo‑A was detected in immersion fixed frozen sections of rat embryo (E18) using Mouse Anti-Rat Nogo‑A Monoclonal Antibody (Catalog # MAB3098) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to spinal cord and dorsal root ganglia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-A

Nogo-A, also known as Reticulon-4, is the longest of four splice variants of Nogo. It is a CNS myelin-associated neurite outgrowth inhibitor that is highly expressed in oligodendrocytes. Nogo-A is synthesized as an 1163 amino acid protein and lacks a signal peptide. Within conserved C-terminal reticulon homology domain (RHD), two transmembrane domains, which are separated by a 66 amino acid extracellular loop, exist. Both the N-terminal domain and the 66 amino acid domain (Nogo-66) can be detected on the cell surface and show neurite outgrowth inhibitory activity. The amino acid sequence of rat Nogo-A N-terminal domain is 76% identical to that of human Nogo-A. Within the RHD domain, rat Nogo-A shares 99% and 97% amino acid sequence identity with mouse and human Nogo, respectively.

  • Long Name:
    Reticulon 4A
  • Entrez Gene IDs:
    57142 (Human); 68585 (Mouse); 83765 (Rat)
  • Alternate Names:
    NI220; NogoA; Nogo-A; RTN4; RTN4A
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