Rat ROBO1 Antibody

Catalog # Availability Size / Price Qty
AF1749
AF1749-SP
ROBO1 in Rat Embryo.
2 Images
Product Details
Citations (7)
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Rat ROBO1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat ROBO1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat ROBO1
Lys19-Ile560
Accession # O55005
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Rat ROBO1 Fc Chimera (Catalog # 1749-RB)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry ROBO1 in Rat Embryo. View Larger

ROBO1 in Rat Embryo. ROBO1 was detected in immersion fixed frozen sections of rat embryo (neural tube) using 5 µg/mL Goat Anti-Rat ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1749) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry ROBO1 in Rat Brain. View Larger

ROBO1 in Rat Brain. ROBO1 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Rat ROBO1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1749) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROBO1

Rat ROBO1 (also DUTT1) is a 170-200 kDa member of the four molecule ROBO family of guidance molecules (1-3). The term ROBO derives from round-about, a description of the circuitous pathway axons take in the absence of a functional ROBO gene (3, 4). Rat ROBO1 is a type I transmembrane (TM) glycoprotein that is synthesized as a 1651 amino acid (aa) precursor. It contains an 18 aa signal sequence, an 879 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 733 aa cytoplasmic region (5, 6). The ECD contains five C2-type Ig-like domains (aa 68-541) and three fibronectin (FN) type III domains (aa 561-864). The cytoplasmic region contains multiple 15-20 aa long CC (conserved cytoplasmic) motifs (C0-C3) (7, 8). Rat ROBO1 is likely to have at least one isoform. Based on the human and mouse gene, rat ROBO1 will utilize an alternate start site, creating an A (long) and B (short) isoform. The difference is the presence of a 32 aa extension at the N-terminus of the mature molecule (occurs in the A form) (9-12). Based on ROBO3 studies, this extension will impact the ability of ROBO1 to bind Slit (10). Rat ROBO1 ECD is 98% and 96% aa identical to the ECD in mouse and human ROBO1, respectively. ROBO1 serves as a repulsing molecule for axons that cross the midline. Initially, ROBO3 allows outgrowing axons to traverse the midline/floorplate. Once crossed, axons express ROBO1 which deflects neurites attempting to recross to the ipsilateral side (13). The chemorepulsant activity of ROBO1 is dependent on ROBO1 binding to SLIT1/2. Inhibition of ROBO1 is likely due to ROBO1-ROBO3 heterophilic binding (10, 13-15).

References
  1. Guthrie, S. (2001) Curr. Biol. 11:R300.
  2. Guthrie, S. (2004) Curr. Biol. 14:R632.
  3. Park, K.W. et al. (2003) Dev. Biol. 261:251.
  4. Seeger, M. et al. (1993) Neuron 10:409.
  5. Kidd, T. et al. (1998) Cell 92:205.
  6. Marillat, V. et al. (2002) J. Comp. Neurol. 442:130.
  7. Bashaw, G.J. et al. (2000) Cell 101:703.
  8. Jen, J.C. et al. (2004) Science 304:1509.
  9. Clark, K. et al. (2002) FEBS Lett. 523:12.
  10. Camurri, L. et al. (2005) Mol. Cell. Neurosci. 30:485.
  11. Sundaresan, V. et al. (1998) Mol. Cell. Neurosci. 11:29.
  12. GenBank Accession # O55005.
  13. Sabatier, C. et al. (2004) Cell 117:157.
  14. Liu, Z. et al. (2004) Mol. Cell. Neurosci. 26:232.
  15. Brose, K. et al. (1999) Cell 96:795.
Long Name
Roundabout Homolog 1
Entrez Gene IDs
6091 (Human); 58946 (Rat)
Alternate Names
DUTT1; DUTT1Drosophila) homolog 1; H-Robo-1; MGC131599; MGC133277; ROBO1; roundabout, axon guidance receptor, homolog 1 (Drosophila)

Product Datasheets

Citations for Rat ROBO1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Floor-plate-derived netrin-1 is dispensable for commissural axon guidance
    Authors: C Dominici, JA Moreno-Bra, SR Puiggros, Q Rappeneau, N Rama, P Vieugue, A Bernet, P Mehlen, A Chédotal
    Nature, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Spontaneous activity regulates Robo1 transcription to mediate a switch in thalamocortical axon growth.
    Authors: Mire E, Mezzera C, Leyva-Diaz E, Paternain AV, Squarzoni P, Bluy L, Castillo-Paterna M, Lopez MJ, Peregrin S, Tessier-Lavigne M, Garel S, Galceran J, Lerma J, Lopez-Bendito G
    Nat. Neurosci., 2012;15(8):1134-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Robo1 regulates semaphorin signaling to guide the migration of cortical interneurons through the ventral forebrain.
    Authors: Hernandez-Miranda LR, Cariboni A, Faux C, Ruhrberg C, Cho JH, Cloutier JF, Eickholt BJ, Parnavelas JG, Andrews WD
    J. Neurosci., 2011;31(16):6174-87.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  4. Robos and slits control the pathfinding and targeting of mouse olfactory sensory axons.
    Authors: Nguyen-Ba-Charvet KT, Di Meglio T, Fouquet C, Chedotal A
    J. Neurosci., 2008;28(16):4244-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Pioneer longitudinal axons navigate using floor plate and Slit/Robo signals.
    Authors: Farmer WT, Altick AL, Nural HF, Dugan JP, Kidd T, Charron F, Mastick GS
    Development, 2008;135(22):3643-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Fresh
  6. Manipulating Robo expression in vivo perturbs commissural axon pathfinding in the chick spinal cord.
    Authors: Reeber SL, Sakai N, Nakada Y, Dumas J, Dobrenis K, Johnson JE, Kaprielian Z
    J. Neurosci., 2008;28(35):8698-708.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  7. The role of Slit-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons.
    Authors: Andrews W, Barber M, Hernadez-Miranda LR, Xian J, Rakic S, Sundaresan V, Rabbitts TH, Pannell R, Rabbitts P, Thompson H, Erskine L, Murakami F, Parnavelas JG
    Dev. Biol., 2007;313(2):648-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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