Rat TrkA Antibody
Rat TrkA Antibody Summary
Ala33-Pro418
Accession # P35739
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rat TrkA by Western Blot. Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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TrkA in Rat Dorsal Root Ganglion. TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkA
TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.
- Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
- Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Product Datasheets
Citations for Rat TrkA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain
Authors: DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha
Oncogene, 2022;12(1):9241.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch
Authors: K Kanehisa, K Koga, S Maejima, Y Shiraishi, K Asai, M Shiratori-, MF Xiao, H Sakamoto, PF Worley, M Tsuda
Nature Communications, 2022;13(1):2367.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons
Authors: S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic
Frontiers in Physiology, 2021;12(0):644929.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation
Authors: MA Landy, M Goyal, KM Casey, C Liu, HC Lai
Cell Reports, 2021;34(13):108913.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis
Authors: K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato
Journal of pharmacological sciences, 2021;146(3):125-135.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
Cell Rep, 2020;33(8):108425.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates
Authors: L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab
Nat Commun, 2020;11(1):4175.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain
Authors: M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer
J Neuroinflammation, 2020;17(1):183.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates
Authors: L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab
Nat Commun, 2020;11(1):4175.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone
Authors: M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic
Molecular pain, 2020;16(0):1744806920975.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity
Authors: X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang
Cell Rep, 2019;27(13):3799-3807.e3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis
Authors: L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast
Cell Rep, 2019;26(13):3484-3492.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis
Authors: H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao
Nat Commun, 2019;10(1):181.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
Authors: S Wiszniak, Q Schwarz
BMC Neurosci, 2019;20(1):21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F -
A cell fitness selection model for neuronal survival during development
Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
Nat Commun, 2019;10(1):4137.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats
Authors: R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda
eNeuro, 2018;5(1):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur
Authors: SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh
Neuroscience, 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors
Authors: I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner
Mol Pain, 2018;14(0):1744806918814.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
[EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur
Authors: SR Chartier, SA Mitchell, LA Majuta, P Mantyth
Mol Pain, 2017;0(0):1744806917745.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
J. Neurosci. Methods, 2017;282(0):34-42.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
Nat Neurosci, 2015;18(1):145-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
Authors: Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S
J Neurosci, 2014;34(4):1494-509.
Species: Rat
Sample Types: Whole Cells
Applications: IHC -
Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
Authors: Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D
BMC Neurosci, 2013;15(0):1.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
J Biol Chem, 2013;288(16):11144-54.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
J. Mol. Cell. Cardiol., 2010;49(1):79-87.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
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E12 mouse DRG neurons grown overnight with 10ng/ml NGF, then fixed in 4% PFA, blocked for 1 h with 5% BSA, and incubated overnight in 1% BSA containing AF1056 (1:300). A fluorophore-conjugated secondary antibody was used to visualise reactivity.
