Rat TrkA Antibody
Rat TrkA Antibody Summary
Ala33-Pro418
Accession # P35739
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
View Larger
Detection of Rat TrkA by Western Blot. Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
TrkA in Rat Dorsal Root Ganglion. TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TrkA
TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.
- Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
- Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Product Datasheets
Citations for Rat TrkA Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
15
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone
Authors: M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic
Molecular pain, 2020;16(0):1744806920975.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis
Authors: L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast
Cell Rep, 2019;26(13):3484-3492.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis
Authors: H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao
Nat Commun, 2019;10(1):181.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
Authors: S Wiszniak, Q Schwarz
BMC Neurosci, 2019;20(1):21.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F -
A cell fitness selection model for neuronal survival during development
Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
Nat Commun, 2019;10(1):4137.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors
Authors: I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner
Mol Pain, 2018;14(0):1744806918814.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur
Authors: SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh
Neuroscience, 2018;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats
Authors: R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda
eNeuro, 2018;5(1):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
J. Neurosci. Methods, 2017;282(0):34-42.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
[EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur
Authors: SR Chartier, SA Mitchell, LA Majuta, P Mantyth
Mol Pain, 2017;0(0):1744806917745.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
Nat Neurosci, 2015;18(1):145-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
Authors: Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S
J Neurosci, 2014;34(4):1494-509.
Species: Rat
Sample Types: Whole Cells
Applications: IHC -
An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
J Biol Chem, 2013;288(16):11144-54.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
Authors: Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D
BMC Neurosci, 2013;15(0):1.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
J. Mol. Cell. Cardiol., 2010;49(1):79-87.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsCell and Tissue Staining Kits
Immunohistochemistry Reagents
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Rat TrkA Antibody
Average Rating: 4.5 (Based on 2 Reviews)
Have you used Rat TrkA Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
E12 mouse DRG neurons grown overnight with 10ng/ml NGF, then fixed in 4% PFA, blocked for 1 h with 5% BSA, and incubated overnight in 1% BSA containing AF1056 (1:300). A fluorophore-conjugated secondary antibody was used to visualise reactivity.
