Recombinant Human Common gamma Chain Fc Avi Protein, CF

Name: Recombinant Human Common gamma Chain Fc Avi Protein, CF
Brand: R&D Systems
SKU: AVI11045
Price: 642 USD
Availability: InStock

R&D Systems | Catalog # AVI11045

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Product Documents

Biotinylated IL-2 R gamma Fc Chimera Avi-tag

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems CHO-derived Recombinant Human Common gamma Chain Fc Avi Protein (AVI11045)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

P31785.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Common gamma Chain/IL-2 R gamma Proteins and Enzymes »

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Common gamma Chain/IL-2 R gamma protein

Human IL-2RG (Leu23-Asn254) Accession # P31785.1	IEGRMD	Human IgG₁ Fc (Pro100-Lys330)	Avi-tag
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu23

Predicted Molecular Mass

56 kDa

SDS-PAGE

85-95 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD25/IL-2R alpha Protein (Catalog # 223-2A) and Recombinant Human IL-2R beta Protein (Catalog # 223-2B) are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi‑tag (Catalog # AVI11045) that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL‑2 Protein (Catalog # 202-IL).

Scientific Data Images for Recombinant Human Common gamma Chain Fc Avi Protein, CF

Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human CD25/IL-2R alpha Protein ( 223-2A) and Recombinant Human IL-2R beta Protein (223-2B) are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi-tag Protein (Catalog # AVI11045) that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL-2 Protein (202-IL).

Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi-tag Protein (Catalog # AVI11045) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-95 kDa and 170-190 kDa, respectively.

Formulation, Preparation, and Storage

AVI11045

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma _c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene. Our Avi-tag Biotinylated IL-2 R gamma features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.