Recombinant Human Common gamma Chain Fc Avi Protein, CF

Biotinylated IL-2 R gamma Fc Chimera Avi-tag
Catalog # Availability Size / Price Qty
AVI11045-050

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Biotinylated Recombinant Human Common  gamma  Chain/IL‑2 R gamma  Fc Chimera Avi-tag Protein Binding Activity.
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Recombinant Human Common gamma Chain Fc Avi Protein, CF Summary

Learn more about Avi-tag Biotinylated Proteins

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CD25/IL-2R alpha Protein (Catalog # 223-2A) and Recombinant Human IL-2R beta Protein (Catalog # 223-2B) are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common  gamma  Chain/IL‑2 R gamma Fc Chimera Avi‑tag (Catalog # AVI11045) that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL‑2 Protein (Catalog # 202-IL).
Source
Chinese Hamster Ovary cell line, CHO-derived human Common gamma Chain/IL-2 R gamma protein
Human IL-2RG
(Leu23-Asn254)
Accession # P31785.1
IEGRMDHuman IgG1 Fc
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu23
Structure / Form
Disulfide-linked homodimer
Biotinylated via Avi-tag
Predicted Molecular Mass
56 kDa
SDS-PAGE
85-95 kDa, under reducing conditions.

Product Datasheets

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AVI11045

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11045

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

When Recombinant Human CD25/IL-2R alpha Protein ( 223-2A) and Recombinant Human IL-2R beta Protein (223-2B) are immobilized at 5.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Common  gamma  Chain/IL‑2 R gamma Fc Chimera Avi-tag Protein (Catalog # AVI11045) that produces 50% of the optimal binding response is 0.250-2.50 μg/mL in the presence of Recombinant Human IL-2 Protein (202-IL).

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human Common gamma Chain/IL‑2 R gamma Fc Chimera Avi-tag Protein (Catalog # AVI11045) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-95 kDa and 170-190 kDa, respectively.

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Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gamma c). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene. Our Avi-tag Biotinylated IL-2 R gamma  features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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