SARS-CoV-2 ORF3a Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of SARS-CoV-2 ORF3a by Western Blot. Western blot shows recombinant SARS-CoV-2 ORF3a peptide. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ORF3a at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
ORF3a in HEK293 Human Cell line Transfected with SARS-CoV-2 ORF3a. ORF3a was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Mouse Anti-SAR-CoV-2 ORF3a Monoclonal Antibody (Catalog # MAB10706) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of SARS-CoV-2 ORF3a by Simple WesternTM. Simple Western lane view shows recombinant SARS-CoV-2 ORF3a, loaded at 0.2 mg/mL. A specific band was detected for ORF3a at approximately 34 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ORF3a is an open reading frame coronavirus protein involved in virus replication in release. Analysis of ORF3a nucleotide and protein sequences can predict their ability to alter viral cycle and provides insight into the biology of coronaviruses. ORF3a has been characterized in both SARS-CoV which caused the SARS outbreak and in SARS-CoV-2 which caused the COVID-19 pandemic. ORF3a is a high priority target that is amenable to drug treatment for COVID-19 post viral syndrome. Mutations in ORF3a can help understand viral virulence and design suitable therapeutics.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for SARS-CoV-2 ORF3a Antibody
There are currently no reviews for this product. Be the first to review SARS-CoV-2 ORF3a Antibody and earn rewards!
Have you used SARS-CoV-2 ORF3a Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image