Key Product Details

Species Reactivity

Validated:

SARS-CoV-2

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1035921
View all ORF3a Primary Antibodies »

Product Specifications

Immunogen

SARS-CoV-2 ORF3a peptide

Specificity

Detects SARS-CoV-2 ORF3a in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SARS-CoV-2 ORF3a Antibody

Detection of SARS-CoV-2 ORF3a by Western Blot.

Western blot shows recombinant SARS-CoV-2 ORF3a peptide. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ORF3a at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

ORF3a in HEK293 Human Cell line Transfected with SARS-CoV-2 ORF3a.

ORF3a was detected in immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a (positive staining) and HEK293 human embryonic kidney cell line (non-transfected, negative staining) using Mouse Anti-SAR-CoV-2 ORF3a Monoclonal Antibody (Catalog # MAB10706) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of SARS-CoV-2 ORF3a by Simple WesternTM.

Simple Western lane view shows recombinant SARS-CoV-2 ORF3a, loaded at 0.2 mg/mL. A specific band was detected for ORF3a at approximately 34 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human ORF3a Monoclonal Antibody (Catalog # MAB10706). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for SARS-CoV-2 ORF3a Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HEK293 human embryonic kidney cell line transfected with SARS-CoV-2 ORF3a

Simple Western

20 µg/mL
Sample: Recombinant SARS-CoV-2 ORF3a

Western Blot

1 µg/mL
Sample: Recombinant SARS-CoV-2 ORF3a

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ORF3a

ORF3a is an open reading frame coronavirus protein involved in virus replication in release.  Analysis of ORF3a nucleotide and protein sequences can predict their ability to alter viral cycle and provides insight into the biology of coronaviruses.  ORF3a has been characterized in both SARS-CoV which caused the SARS outbreak and in SARS-CoV-2 which caused the COVID-19 pandemic.  ORF3a is a high priority target that is amenable to drug treatment for COVID-19 post viral syndrome.  Mutations in ORF3a can help understand viral virulence and design suitable therapeutics.

Long Name

ORF3a

Alternate Names

U274, X1

Entrez Gene IDs

43740569 (SARS-CoV-2)

Gene Symbol

ORF3A

Additional ORF3a Products

Product Documents for SARS-CoV-2 ORF3a Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for SARS-CoV-2 ORF3a Antibody

For research use only

Related Research Areas

Infectious Diseases

Citations for SARS-CoV-2 ORF3a Antibody

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Protocols

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