Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Tau Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Tau
Gln624-Gln756
Accession # P10636

Specificity

Detects human Tau in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Tau Antibody

Tau antibody in Human Alzheimer's Disease Brain by Immunohistochemistry (IHC-P).

Tau in Human Alzheimer's Disease Brain.

Tau was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 5 µg/mL Goat Anti-Tau Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3494) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Tau Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Tau

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tau

Tau is a microtubule-associated protein primarily expressed in neurons. It can associate with microtubules through the carboxy-terminal domains and with the plasma membrane through the amino-terminal projection domain. Tau has a role as a stabilizer of microtubules. Abnormal Tau phosphorylation or splicing is associated with various neurological disorders.

Long Name

Microtubule-Associated Protein Tau

Alternate Names

MAPT, MSTD, MTBT1, Neurofibrillary tangle protein, PPND

Entrez Gene IDs

4137 (Human); 17762 (Mouse); 29477 (Rat)

Gene Symbol

MAPT

UniProt

P10636

Additional Tau Products

Product Documents for Tau Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Tau Antibody

For research use only

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Protocols

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