Tau Antibody

Catalog #: AF3494
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3494
AF3494-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Tau Antibody Summary

Specificity

Detects human Tau in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Tau
Gln624-Gln756
Accession # P10636

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human Tau

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Tau in Human Alzheimer's Disease Brain. Tau was detected in immersion fixed paraffin-embedded sections of human Alzheimer's disease brain (cortex) using 5 µg/mL Goat Anti-Tau Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3494) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tau

Tau is a microtubule-associated protein primarily expressed in neurons. It can associate with microtubules through the carboxy-terminal domains and with the plasma membrane through the amino-terminal projection domain. Tau has a role as a stabilizer of microtubules. Abnormal Tau phosphorylation or splicing is associated with various neurological disorders.

Long Name

Microtubule-Associated Protein Tau

Entrez Gene IDs

4137 (Human); 17762 (Mouse); 29477 (Rat)

Alternate Names

DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MGC138549; microtubule-associated protein tau; MSTD; MSTDMAPTL; MTBT1; MTBT1Neurofibrillary tangle protein; MTBT2; Neurofibrillary tangle protein; PHF-tau; PPND; Tau; TAUPaired helical filament-tau

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Tau Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Amyloid Beta and Tau Cooperate to Cause Reversible Behavioral and Transcriptional Deficits in a Model of Alzheimer's Disease
Authors: EK Pickett, AG Herrmann, J McQueen, K Abt, O Dando, J Tulloch, P Jain, S Dunnett, S Sohrabi, MP Fjeldstad, W Calkin, L Murison, RJ Jackson, M Tzioras, A Stevenson, M d'Orange, M Hooley, C Davies, M Colom-Cade, A Anton-Fern, D King, I Oren, J Rose, CA McKenzie, E Allison, C Smith, O Hardt, CM Henstridge, GE Hardingham, TL Spires-Jon
Cell Rep, 2019;29(11):3592-3604.e5.
Species: Human
Sample Types: Whole Tissue
Applications: IHC