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Viral Chikungunya Virus E1 Antibody

Catalog #: MAB97792
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB97792-100
MAB97792-SP

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Detection of Chikungunya Virus E1 by Western Blot.
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Viral Chikungunya Virus E1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral Chikungunya Virus E1 in ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 988122
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1
Tyr810-Gly1224
Accession # Q8JUX5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Chikungunya Virus E1 antibody by Western Blot. View Larger

Detection of Chikungunya Virus E1 by Western Blot. Western blot shows lysates of recombinant Chikungunya Virus (CHIKV) E1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97792) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chikungunya Virus E1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chikungunya Virus E1

Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.

Long Name
Chikungunya Virus Envelope 1
Entrez Gene IDs
956308 (Viral)
Alternate Names
CHICKV E1; Chikungunya Virus E1; Chikungunya Virus; CHIKV-E1

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Citation for Viral Chikungunya Virus E1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Chikungunya virus entry and infectivity is primarily facilitated through cell line dependent attachment factors in mammalian and mosquito cells
    Authors: Judith Mary Reyes Ballista, Kerri L. Miazgowicz, Marissa D. Acciani, Ariana R. Jimenez, Ryan S. Belloli, Katherine E. Havranek et al.
    Frontiers in Cell and Developmental Biology

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