Key Product Details

Species Reactivity

Validated:

Viral

Cited:

Human, Primate - Chlorocebus aethiops (African Green Monkey)

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 988122
View all Chikungunya Virus E1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived transfected with viral Chikungunya Virus E1
Tyr810-Gly1224
Accession # Q8JUX5

Specificity

Detects viral Chikungunya Virus E1 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Viral Chikungunya Virus E1 Antibody

Detection of Chikungunya Virus E1 antibody by Western Blot.

Detection of Chikungunya Virus E1 by Western Blot.

Western blot shows lysates of recombinant Chikungunya Virus (CHIKV) E1. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral Chikungunya Virus E1 Monoclonal Antibody (Catalog # MAB97792) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Chikungunya Virus E1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Viral Chikungunya Virus E1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Recombinant Chikungunya Virus E1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chikungunya Virus E1

Chikungunya fever is a pandemic disease caused by the flavivirus Chikungunya (CHIKV). The 47 kDa E1 glycoprotein mediates viral membrane fusion during CHIKV infection. The CHIKV envelope protein E1 is a component of the viral spike, which is composed of triplets of heterodimer of E1 and E2 glycoproteins, expressed on the viral surface. The viral spike proteins facilitate attachment to cell surfaces and viral entry into the cells. The E1 envelope protein is a class II fusion protein that mediates low pH-triggered membrane fusion during virus infection.

Long Name

Chikungunya Virus Envelope 1

Alternate Names

CHICKV E1, CHIKV-E1

Entrez Gene IDs

956308 (Viral)

Gene Symbol

CHIKVGP2

UniProt

Q8JUX5

Additional Chikungunya Virus E1 Products

Product Documents for Viral Chikungunya Virus E1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Viral Chikungunya Virus E1 Antibody

For research use only

Related Research Areas

Infectious Diseases

Citations for Viral Chikungunya Virus E1 Antibody

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