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Viral HCMV IL-10 Antibody

Catalog #: AF117
10 Citations Datasheet / COA / SDS
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AF117
AF117-SP
IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Viral HCMV IL-10 Antibody Summary

Species Reactivity
Viral
Specificity
Detects viral HCMV IL-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑10, recombinant canine IL‑10, recombinant feline IL‑10, recombinant cotton rat IL‑10, recombinant rat IL‑10, recombinant porcine IL‑10, recombinant human IL‑10, and recombinant Epstein-Barr virus encoded viral IL‑10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant viral HCMV IL‑10
Ala26-Lys176
Accession # AAF63437
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Viral HCMV IL‑10 (Catalog # 117-VL)
Neutralization
Measured by its ability to neutralize IL‑10-mediated inhibition of LPS-induced IL-1 beta secretion in human peripheral blood lymphocytes (PBL). The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 2 ng/mL Recombinant Viral HCMV IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. View Larger

IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. Recombinant Viral HCMV IL-10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND50 is typically 0.15-0.9 µg/mL.

Western Blot Detection of Viral HCMV IL-10 by Western Blot View Larger

Detection of Viral HCMV IL-10 by Western Blot LAcmvIL-10 and cmvIL-10 suppress tumor proliferation, migration, invasion, and colony formation in vitro. a The expression of recombinant LAcmvIL-10 and cmvIL-10 in GC cell lines by western blotting. GAPDH was used as an internal control. The cell proliferation (b), colony formation (c), and migration and invasion (d) of GC cells were examined. Error bars represent the standard error of the mean obtained from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32025866), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).

References
  1. Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
  2. Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
  3. Spencer, J.V. et al. (2002) J. Virol. 76:1285.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 100034187 (Equine); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Viral HCMV IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Cytomegalovirus proteins, maternal pregnancy cytokines, and their impact on neonatal immune cytokine profiles and acute lymphoblastic leukemogenesis in children
    Authors: Joseph L. Wiemels, Rong Wang, Mi Zhou, Helen Hansen, Rachel Gallant, Junghyun Jung et al.
    Haematologica
    Species: Human
    Sample Types: Serum, Whole Blood
    Applications: ELISA Capture
  2. Challenging the Conventional Interpretation of HCMV Seronegativity
    Authors: S Waters, S Lee, A Irish, P Price
    Microorganisms, 2021-11-18;9(11):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Capture
  3. Common Variable Immunodeficiency patients with a phenotypic profile of immunosenescence present with thrombocytopenia
    Authors: J Stuchl?, V Kanderov , M Vlkov , I He?manov , L Sl mov , O Pel k, E Taraldsrud, D J¡lek, P Kr l¡c Kov, B Fevang, M Trkov , O Hru? k, E Fro?kov , A ?ediv , J Litzman, T Kalina
    Sci Rep, 2017-01-05;7(0):39710.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  4. cmvIL-10 stimulates the invasive potential of MDA-MB-231 breast cancer cells.
    Authors: Valle Oseguera, Cendy A, Spencer, Juliet V
    PLoS ONE, 2014-02-10;9(2):e88708.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct.
    Authors: Forster S, Brandt M, Mottok D, Zschuttig A, Zimmermann K, Blattner F, Gunzer F, Pohlmann C
    BMC Biotechnol, 2013-10-05;13(0):82.
    Species: Virus
    Sample Types: Cell Fraction
    Applications: ELISA Development, Western Blot
  6. Gliomas Promote Immunosuppression through Induction of B7-H1 Expression in Tumor-Associated Macrophages
    Authors: Orin Bloch, Courtney A. Crane, Rajwant Kaur, Michael Safaee, Martin J. Rutkowski, Andrew T. Parsa
    Clinical Cancer Research
  7. Antibodies to human IL-10 neutralize ebvIL-10-mediated cytokine suppression but have no effect on cmvIL-10 activity
    Authors: Noelle D. Brodeur, Juliet V. Spencer
    Virus Research
  8. Immunomodulatory properties of a viral homolog of human interleukin-10 expressed by human cytomegalovirus during the latent phase of infection.
    Authors: Jenkins C, Garcia W, Godwin MJ, Spencer JV, Stern JL, Abendroth A, Slobedman B
    J. Virol., 2008-01-23;82(7):3736-50.
    Species: Virus
    Sample Types: Recombinant Protein
    Applications: Western Blot
  9. Human cytomegalovirus-encoded interleukin-10 homolog inhibits maturation of dendritic cells and alters their functionality.
    Authors: Chang WL, Baumgarth N, Yu D, Barry PA
    J. Virol., 2004-08-01;78(16):8720-31.
    Species: Virus
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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