Detects viral HCMV IL-10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑10, recombinant canine IL‑10, recombinant feline IL‑10, recombinant cotton rat IL‑10, recombinant rat IL‑10, recombinant porcine IL‑10, recombinant human IL‑10, and recombinant Epstein-Barr virus encoded viral IL‑10 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant viral HCMV IL‑10 Ala26-Lys176 Accession # AAF63437
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize IL‑10-mediated inhibition of LPS-induced IL-1 beta secretion in human peripheral blood lymphocytes (PBL). The Neutralization Dose (ND50) is typically 0.15-0.9 μg/mL in the presence of 2 ng/mL Recombinant Viral HCMV IL‑10.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL-1 beta Secretion Inhibited by IL‑10 and Neutralization by Viral IL‑10 Antibody. Recombinant Viral HCMV IL‑10 (Catalog # 117-VL) inhibits LPS-indecued IL-1 beta secretion in human peripheral blood lymphocytes (PBL) in a dose-dependent manner (orange line). IL-1 beta secretion inhibited by Recombinant Viral HCMV IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Viral HCMV IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF117). The ND50 is typically 0.15-0.9 μg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a crucial role in regulating immune and inflammstory responses (1). It has closely related homologs in the genome of several viruses including the human cytomegalovirus (HCMV), a prevalent pathogen that can remain in humans as a latent infection (2). Viruses use multiple strategies to avoid detection and clearance by the host immune system. One of these strategies is to express virus-encoded host cytokine homologs, such as IL-10, to modulate the host immune system to the virus’ advantage. HCMV IL-10 is encoded from noncontiguous stretches in the viral genome and is transcribed early during infection. It shares 27% amino acid sequence identity with the endogenous human IL-10. Despite its low homology with the human protein, it can bind to the human IL-10 receptor complex and induces signal transduction. HCMV IL-10 has been shown to suppress cytokine production and inhibit proliferation of mitogen-stimulated human peripheral blood mononuclear cells. HCMV IL-10 can also down regulate the antigen-presenting and accessory cell functions of human monocytes. These immunosuppressive properties of HCMV IL-10 likely contribute to immune evasion during virus infection (3).
Moore, K.W. et al. (2001) Annu. Rev. Immunol. 19:683.
Kotenko, S.V. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1695.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.