Viral Yellow Fever Virus NS1 Antibody

Catalog # Availability Size / Price Qty
MAB9776-100
MAB9776-SP
Detection of Yellow Fever Virus NS1 by Western Blot.
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Viral Yellow Fever Virus NS1 Antibody Summary

Species Reactivity
Viral
Specificity
Detects Yellow Fever Virus NS1 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 994706
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell HEK293-derived Yellow Fever Virus NS1
Gln780-His1134
Accession # P03314
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

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Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant viral Yellow Fever Virus NS1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Yellow Fever Virus NS1 by Western Blot. Western blot shows recombinant viral Yellow Fever Virus NS1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB9776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Yellow Fever Virus NS1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

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Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Yellow Fever Virus NS1

Yellow fever virus (YFV) non-structural protein 1 (NS1) is produced by yellow fever virus (YFV), a mosquito-borne flavivirus that is responsible for numerous epidemics of yellow fever disease with high fatality rates (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane‑associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral YFV NS1 contains 352 amino acids (afa) and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

References
  1. Beasley, D.W. et al. (2015) Antiviral. Res. 115:48.
  2. Rastogi, M. et al. (2016) Virol. J. 13:131.
Entrez Gene IDs
1502173 (Viral)
Alternate Names
Yellow Fever Virus NS1; YFV NS1; YFV_NS1; YFV-NS1

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