Viral Yellow Fever Virus NS1 Antibody Summary
Accession # P03314
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Yellow Fever Virus NS1 by Western Blot. Western blot shows recombinant viral Yellow Fever Virus NS1. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Viral Yellow Fever Virus NS1 Monoclonal Antibody (Catalog # MAB9776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Yellow Fever Virus NS1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Yellow Fever Virus NS1
Yellow fever virus (YFV) non-structural protein 1 (NS1) is produced by yellow fever virus (YFV), a mosquito-borne flavivirus that is responsible for numerous epidemics of yellow fever disease with high fatality rates (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane‑associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral YFV NS1 contains 352 amino acids (afa) and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.
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