Viral Zika Virus (H/PF/2013) NS1 Antibody (990907)

R&D Systems | Catalog # MAB99201

R&D Systems

Key Product Details

Species Reactivity

Viral

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 990907
View all Zika Virus (H/PF/2013) NS1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived recombinant Viral Zika Virus (H/PF/2013) NS1
Val796 & Ser799-Ser1148, with a C-terminal 6-His tag
Accession # AHZ13508

Specificity

Detects Viral Zika Virus (H/PF/2013) NS1 in direct ELISAs

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Viral Zika Virus (H/PF/2013) NS1 Antibody (990907)

Viral Zika Virus (H/PF/2013) NS1 Antibody in ELISA Standard Curve.

Viral Zika Virus (H/PF/2013) NS1 ELISA Standard Curve.

Recombinant Viral Zika Virus (H/PF/2013) NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB99201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB9920) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Viral Zika Virus (H/PF/2013) NS1 Antibody (990907)

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB9920).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Zika Virus (H/PF/2013) NS1

Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host.  ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.

References

  1. Song, H. et al. (2016) Nat. Struct. and Mol. Biol. 23:456.
  2. Brown, WC. et al. (2016) Nat. Struct. and Mol. Biol. 23: 865.
  3. Baronti, C. et al. (2014) Genome Announc. 2: e00500

Long Name

Zika Virus Polyprotein

UniProt

AHZ13508

Additional Zika Virus (H/PF/2013) NS1 Products

Product Documents for Viral Zika Virus (H/PF/2013) NS1 Antibody (990907)

Certificate of Analysis

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Product Specific Notices for Viral Zika Virus (H/PF/2013) NS1 Antibody (990907)

For research use only

Related Research Areas

Infectious Diseases

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