Viral Zika Virus (H/PF/2013) NS1 Antibody Summary
Val796 & Ser799-Ser1148, with a C-terminal 6-His tag
Accession # AHZ13508
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB99201).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Viral Zika Virus (H/PF/2013) NS1 ELISA Standard Curve. Recombinant Viral Zika Virus (H/PF/2013) NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB99201) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Zika Virus (H/PF/2013) NS1 Monoclonal Antibody (Catalog # MAB9920) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Zika Virus (H/PF/2013) NS1
Zika Virus non-structural protein 1 (ZIKV NS1) is an approximately 48 kDa viral glycoprotein (1). Zika Virus is a mosquito-borne flavivirus and has been implicated an association with neonatal microcephaly and neurological disorders such as Guillain-Barré syndrome (2). ZIKV strain H/PF/2013 was isolated from human host. ZIKV NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (1, 2). Infected cells secrete NS1 as a lipoprotein, which is involved in immune evasion and pathogenesis via interaction with components of the innate and adaptive immune systems (1, 2). Mature ZIKV NS1 contains 352 amino acids (aa) and has a hexameric conformation consisting of three dimers to form a symmetric barrel shape (1). It has high structural similarity to other flavivirus NS1 proteins, such as DENV and WNV (2, 3). Mature NS1 of ZIKV strain H/PF/2013 shares 98% aa sequence identity with NS1 of ZIKV strain MR 766 that was isolated from monkey host.
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