Canine IL-1 beta /IL-1F2 Antibody Summary
Accession # ABB73214
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutral-ization by Canine IL-1 beta /IL-1F2 Antibody. Recombinant Canine IL-1 beta /IL-1F2 (Catalog # 3747-CL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-1 beta /IL-1F2 (20 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3747). The ND50 is typically 2-10 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 22% amino acid (aa) identity in dog. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL‑1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The canine IL-1 beta cDNA encodes a 266 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature canine IL-1 beta shares 68-78% aa sequence identity with cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus macaque IL-1 beta.
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Accession # NP_001033060.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Citations for Canine IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Evolutionary loss of inflammasomes in the Carnivora and implications for the carriage of zoonotic infections
Authors: Z Digby, P Tourlomous, J Rooney, JP Boyle, B Bibo-Verdu, RJ Pickering, SJ Webster, TP Monie, LJ Hopkins, N Kayagaki, GS Salvesen, S Warming, L Weinert, CE Bryant
Cell Reports, 2021;36(8):109614.
Sample Types: Whole Cells
Increased expression of interleukin-1beta and its novel splice variant in canine hearts with volume overload.
Authors: Kiczak L, Paslawska U, Bania J, Ugorski M, Sambor I, Kochman A, Blach J, Chelmonska-Soyta A
Sample Types: Tissue Homogenates
Applications: Western Blot
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