Canine IL-4 DuoSet ELISA

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Canine IL-4 ELISA Standard Curve
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Product Details
Citations (8)
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Canine IL-4 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant canine IL-4. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Canine IL-4 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-4

IL-4 (Interleukin-4) is a cytokine that is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. The type I receptor for IL-4, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R? and the common ? chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R? and IL-13 R?1. The type II receptor also transduces IL-13 mediated signals.

Long Name:
Interleukin 4
Entrez Gene IDs:
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names:
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Citations for Canine IL-4 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Immortalised canine buccal epithelial cells' CXCL8 secretion is affected by allergen extracts, Toll-like receptor ligands, IL-17A and calcitriol
    Authors: M Pelst, C Höbart, H de Rooster, B Devriendt, E Cox
    Veterinary research, 2022-09-13;53(1):72.
    Species: Canine
    Sample Types: Cell Culture Supernates
  2. Mycobacterium intracellulare induces a Th17 immune response via M1-like macrophage polarization in canine peripheral blood mononuclear cells
    Authors: S Kim, YS Hyun, HT Park, MK Shin, HS Yoo
    Scientific Reports, 2022-07-12;12(1):11818.
    Species: Canine
    Sample Types: Cell Culture Supernates
  3. Combined IMIG and Immune Ig Attenuate Allergic Responses in Beagle Dogs
    Authors: RM Gorczynski, T Maqbool, G Hoffmann
    J Immunol Res, 2020-04-21;2020(0):2061609.
    Species: Canine
    Sample Types: Cell Culture Supernates
  4. A preliminary study assessing cytokine production following ex vivo stimulation of whole blood with diet in dogs with chronic enteropathy
    Authors: A Kathrani, E Hall
    BMC Vet. Res., 2019-06-04;15(1):185.
    Species: Canine
    Sample Types: Cell Culture Supernates
  5. Neutrophil properties in healthy and Leishmania infantum-naturally infected dogs
    Authors: AB Wardini, LH Pinto-da-S, NR Nadaes, MT Nascimento, BM Roatt, AB Reis, KF Viana, RC Giunchetti, EM Saraiva
    Sci Rep, 2019-04-18;9(1):6247.
    Species: Canine
    Sample Types: Cell Culture Supernates
  6. PD-1 and PD-L1 regulate cellular immunity in canine visceral leishmaniasis
    Authors: KL Oliveira S, V Marin Chik, G Luvizotto, AA Correa Lea, BF de Almeida, F De Rezende, PSP Dos Santos, G Fabrino Ma, VMF De Lima
    Comp. Immunol. Microbiol. Infect. Dis., 2018-12-14;62(0):76-87.
    Species: Canine
    Sample Types: Cell Culture Supernates
  7. Oat beta-glucan as a dietary supplement for dogs
    Authors: LG Ferreira, M Endrighi, KG Lisenko, MRD de Oliveir, MR Damasceno, JA Claudino, PG Gutierres, AP Peconick, FMOB Saad, MG Zangeronim
    PLoS ONE, 2018-07-31;13(7):e0201133.
    Species: Canine
    Sample Types: Plasma
  8. Expression of regulatory T cells in jejunum, colon, and cervical and mesenteric lymph nodes of dogs naturally infected with Leishmania infantum.
    Authors: Figueiredo M, Deoti B, Amorim I, Pinto A, Moraes A, Carvalho C, da Silva S, de Assis A, de Faria A, Tafuri W
    Infect Immun, 2014-06-16;82(9):3704-12.
    Species: Canine
    Sample Types: Tissue Homogenates


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