Cotton Rat IL-4 Antibody

Catalog # Availability Size / Price Qty
MAB584
MAB584-SP
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Cotton Rat IL-4 Antibody Summary

Species Reactivity
Cotton Rat
Specificity
Detects recombinant cotton rat IL-4 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human IL-4, recombinant rat IL-4, recombinant porcine IL-4, recombinant feline IL-4, recombinant canine IL-4, recombinant human IL-4 Ra, or recombinant mouse IL-4 Ra was observed.
Source
Monoclonal Mouse IgG1 Clone # 143514
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant cotton rat IL‑4
Cys21-Phe147
Accession # AAL18820
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Cotton Rat IL‑4 (Catalog # 584-R4) under non-reducing conditions only

Cotton Rat IL-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Cotton Rat IL‑4 Biotinylated Antibody (Catalog # BAF584)

Standard: Recombinant Cotton Rat IL-4 Protein (Catalog # 584-R4)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Cotton rat IL‑4 is synthesized with a 24 amino acid (aa) signal sequence. Mature cotton rat IL‑4 shares 41%, 44%, 57%, and 68% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. IL-4 exerts its effects through two receptor complexes (5, 6). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (7‑10). IL-4 plays a dominant role in the development of allergic inflammation and asthma (9, 11).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Accession # AAL18820.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  6. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  7. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  8. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  9. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  10. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  11. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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