Feline IL-8/CXCL8 Antibody

  
  • Species Reactivity
    Feline
  • Specificity
    Detects feline IL-8/CXCL8 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross‑reactivity with recombinant canine IL-8/CXCL8 is observed and 15% cross-reactivity with recombinant porcine IL-8/CXCL8 and recombinant human IL-8/CXCL8 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant feline IL-8/CXCL8
    Ala23-Ala101
    Accession # Q9XSX5.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL)
  • Immunocytochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize IL-8/CXCL8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 20 ng/mL Recombinant Feline IL-8/CXCL8.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by
IL-8/CXCL8 and Neutral­ization by Feline
IL-8/CXCL8 Antibody.
Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline
IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concen­trations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND50 is typically 0.1‑0.4 µg/mL.
Immunocytochemistry
IL-8/CXCL8 in Feline PBMCs. IL-8/CXCL8 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline
IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-8/CXCL8

Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively.

  • References:
    1. Van Damme, J. et al. (1998) The Cytokine Handbook, A.W. Thomson, ed., Academic Press, NY p. 271.
    2. Heidemann, J. et al. (2003) J. Biol. Chem. 178:8508.
    3. Yang, M.P. et al. (2002) Vet. Immunol. Immunopathol. 86:43.
    4. Parhar, K. et al. (2003) Immunology 108:502.
  • Long Name:
    Interleukin 8
  • Entrez Gene IDs:
    3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
  • Alternate Names:
    3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; MDNCF; MDNCFb-ENAP; member 8; MONAPGCP1; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
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