Chemotaxis Induced by|
IL-8/CXCL8 and Neutralization by Feline
IL-8/CXCL8 Antibody. Recombinant Feline IL-8/CXCL8 (Catalog # 2277-FL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Feline
IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277). The ND50 is typically 0.1‑0.4 µg/mL.
IL-8/CXCL8 in Feline PBMCs. IL-8/CXCL8 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline|
IL-8/CXCL8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2277) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Interleukin 8 (IL-8), also named CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil-activating protein 1 (NAP-1), neutrophil-activating factor (NAF) and granulocyte chemotactic peptide (GCP), belongs to the Glu-Leu-Arg motif containing (ELR+) CXC chemokine family and has been designated CXCL8. IL-8 is a potent neutrophil chemoattractant that recruits neutrophils to sites of inflammation. IL-8 also activates neutrophil functions and promotes angiogenesis. The biological activites of IL-8 is mediated by two types of G protein-coupled chemokine receptors, CXCR1 and CXCR2 (1, 2). In normal tissues, IL-8 expression and secretion is barely detectable. Upon stimulation by a wide range of pro-inflammatory signals including exposure to IL-1, TNF, bacterial or viral products, IL-8 production is rapidly induced in many different cell types (3, 4). Feline IL-8 encodes a 101 amino acid (aa) precursor protein with a putative 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine IL-8, respectively.