Feline SCF/c-kit Ligand Antibody AF2268: R&D Systems

Feline SCF/c-kit Ligand Antibody

  
  • Species Reactivity
    Feline
  • Specificity
    Detects feline SCF/c‑kit Ligand in Western blots. In Western blots, approximately 60% cross-reactivity with recombinant mouse SCF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant feline SCF/c‑kit Ligand
    Lys26-Ala190
    Accession # P79169
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Feline SCF/c-kit Ligand (Catalog # 2268-SC)
  • Immunocytochemistry
    10-20 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
SCF/c‑kit Ligand in Feline PBMCs. SCF/c‑kit Ligand was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2268) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCF/c-kit Ligand

Feline SCF (stem cell factor; also known as c-kit ligand) is a type I transmembrane (TM) glycoprotein that plays an important role in a number of fetal and adult developmental processes (1‑4). It is synthesized as a 274 amino acid (aa) precursor that contains a 25 aa signal sequence, a 190 aa extracellular region, a 23 aa TM segment and a 36 aa cytoplasmic tail (5). Within the extracellular region there are two intrachain disulfide bonds and four alpha -helices. Although the predicted molecular weight is 19 kDa, the native molecule is anywhere from 28‑40 kDa in size and reflects both N- and O-linked glycosylation (1). Glycosylation is not necessary for bioactivity (6). The transmembrane form of SCF can be cleaved proteolytically, generating a 165 aa soluble form. Circulating SCF exists as both a monomer and nondisulfide-linked homodimer, with monomer predominating (50% to 75%) (6). Both the soluble and TM forms have bioactivity. Their principal targets may be different, however (7). A second, alternate splice short form of feline SCF has been identified (5). It too, is membrane bound and contains 246 aa residues. It will not give rise to a soluble form, since alternate splicing removes the proteolytic cleavage site used in the long form. The ratio of long form to short form varies tissue to tissue (1). Soluble feline SCF shares 93%, 93%, 90%, 87%, and 78% aa sequence identity with porcine, canine, bovine, human and mouse SCF, respectively. Cells known to express SCF include endothelial cells, fibroblasts and keratinocytes (1).

  • References:
    1. Broudy, V.C. (1997) Blood 90:1345.
    2. Nakagawa, S. and T. Kitoh (2000) Curr. Opin. Hematol. 7:133.
    3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1.
    4. Kang, J. and S.D. Der (2004) Curr. Opin. Immunol. 16:180.
    5. Dunham, S.P. and D.E. Onions (1996) DNA Seq. 6:233.
    6. Hsu, Y-R. et al. (1997) J. Biol. Chem. 272:6406.
    7. Kapur, R. et al. (1998) Blood 91:879.
  • Long Name:
    Stem Cell Factor
  • Entrez Gene IDs:
    4254 (Human); 17311 (Mouse); 60427 (Rat); 403507 (Canine); 493937 (Feline)
  • Alternate Names:
    c-kit Ligand; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; MGFSHEP7; SCF; SCFStem cell factor; SFc-Kit ligand; SLF; steel factor
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