Q: I received this antibody in February 2012 and stored the unopened package immediately at 4C. Today (4/5/2012), I opened the package, placed the vial and centrifuged it briefly. I opened the top and found some thick beige-colored liquid which measured around 50 ul. I don't think I can use the antibody. Could you help me with the problem, please?

A: NBP1-47470 is supplied as ascitic fluid, which frequently has a beige-like color, and this could be what you're seeing in your vial. Otherwise, I'm concerned that the antibody may have been damaged by storing it a 4 C for such a long time. There is a very low concentration of preservative in this antibody, which is why we recommend storage at -20 C long term.

Q: Our customer would like to know whether NBP1-47470, GSK3 beta Antibody can be used in IF. It shows on the website that this antibody can be used to detect the target protein through ICC/IF. But his sampels are paraffin-embedded tissues, no cultured cells. Thus he is not sure. Please give us some advice and help.

A: Our GSK3 beta antibody with catalogue number NBP1-47470 has been validated for IHC with paraffin-embedded tissues, and has also been validated for immunocytochemistry. Our immunocytochemistry data can be seen in image 3 on our website, whilst our IHC-P data can be seen in image 4. This antibody is covered by our 100% quality guarantee for your customer's application of IHC with paraffin-embedded tissues. Currently tested species are human, mouse, rat and primate.

GSK-3 beta Antibody (3D10) - BSA Free

Novus Biologicals | Catalog # NBP1-47470

(4)

Citations (15)

Product Documents

View Available Conjugates View Available Formulations

100% Guarantee

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 3D10

Format

BSA Free

View all GSK-3 beta Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GSK-3 beta Antibody (3D10) - BSA Free

Western Blot: GSK-3 beta Antibody (3D10)BSA Free [NBP1-47470]

Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of GSK-3 beta in mouse beta cell line (betaTC3) using anti-GSK-3 beta antibody. Image from verified customer review.

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470]

Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of NIH/3T3 (left) and U251 (right) cells using GSK3 beta mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470]

GSK-3-beta-Antibody-3D10-Immunohistochemistry-NBP1-47470-img0014.jpg

Flow Cytometry: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470]

Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - An intracellular stain was performed on HeLa cells with GSK-3 beta (3D10) antibody NBP1-47470PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

Western Blot: GSK-3 beta Antibody (3D10)BSA Free [NBP1-47470]

Western Blot: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates.

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470]

Immunohistochemistry-Paraffin: GSK-3 beta Antibody (3D10) [NBP1-47470] - Analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3 beta mouse mAb with DAB staining.

Flow Cytometry: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470]

Flow Cytometry: GSK-3 beta Antibody (3D10) [NBP1-47470] - Flow cytometric analysis of Hela cells using GSK3 beta mouse mAb (green) and negative control (purple).

Simple Western: GSK-3 beta Antibody (3D10)BSA Free [NBP1-47470]

Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple Western lane view shows a specific band for GSK-3 Beta in 0.5 mg/ml of Hek293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: GSK-3 beta Antibody (3D10)BSA Free [NBP1-47470]

Simple Western: GSK-3 beta Antibody (3D10) [NBP1-47470] - Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice. Image courtesy of Dr. Brandi Wasek-Patterson at Baylor Research Institute, Institute of Metabolic Disease.

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] -

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases Fas & Gsk3 beta phosphorylation in CD133+ cells in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases Fas phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Fas or CD133 was performed with DAPI staining of nuclei. (B) SLV peptide increases Gsk3 beta phosphorylation in CD133+ xenograft tumors with or without oxaliplatin. Immunofluorescent detection of phospho-Gsk3 beta or CD133 was performed with DAPI staining of nuclei. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] -

Immunocytochemistry/ Immunofluorescence: GSK-3 beta Antibody (3D10) - BSA Free [NBP1-47470] - Fap1-inhibition with SLV peptide increases phosphorylation of Fap1-substrates Fas & Gsk3 beta in a murine xenograft modelSW620 cells were injected in the flanks of athymic Nude mice & tumor volume was determination biweekly. Mice were treated weekly with oxaliplatin (days 0, 7 & 14) & injected daily with Fap1 blocking SLV peptide or VLS control peptide, or treated with SLV or VLS peptide alone (n=12 per cohort). Tumors were simultaneously harvested from cohorts of mice when control tumors were >2,000 mm3. (A) SLV peptide increases gland formation in xenograft tumors with or without oxaliplatin. Histology was analyzed by hematoxylin/ eosin staining. Fap1 expression was determined by immunofluorescence. Relative fluorescent intensity (RFI) of Fap1 staining is indicated below relevant panels. (B) SLV peptide increases Fas-phosphorylation in xenograft tumors with or without by oxaliplatin. Immunofluorescent detection of total versus phospho-Fas was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. (C) SLV peptide increases Gsk3 beta -phosphorylation with or without oxaliplatin. Immunofluorescent detection of total versus phospho- Gsk3 beta was performed with DAPI staining of nuclei. Areas without gland formation were selected for this study. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29899829), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

View 8 more images

Applications for GSK-3 beta Antibody (3D10) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Simple Western

1:50

Western Blot

1:500-1:2000

Application Notes

This GSK3 beta (3D10) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Mesenchymal stem cells, Hek293 lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 56 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 4 reviews rated 4.8 using NBP1-47470 in the following applications:

Simple Western (2 Reviews)
Western Blot (2 Reviews)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 beta

GSK3 (Glycogen synthase kinase 3), a serine-threonine kinase with two isoforms (alpha and beta), was originally discovered as a key enzyme in glycogen metabolism. GSK3 was subsequently shown to function in cell division, proliferation, motility and survival. GSK3 plays a role in a number of pathological conditions including cancer and diabetes and is increasingly seen as an important component of neurological diseases. GSK3 phosphorylates tau and presenilin-1, which are involved in the development of Alzheimer's disease. Both isoforms of GSK3 are ubiquitously expressed, although particularly high levels of GSK 3 beta are found in the brain where it is involved in synaptic plasticity, possibly via regulation of NMDA receptor trafficking. GSK3 phosphorylates over 40 different substrates including signaling proteins, transcription factors and structural proteins, and is part of the signal transduction cascade of a large number of growth factors and cytokines. The activity of GSK is regulated by phosphorylation (Akt: Akt-mediated phosphorylation at Ser21 of GSK3 alpha and Ser9 of GSK3 beta, S6K, RSK, PKA and PKC), dephosphorylation (PP1 and PP2A), and by binding to protein complexes (with beta-catenin, axin, CK1 and the APC complex).

Long Name

Glycogen Synthase Kinase 3 beta

Alternate Names

GSK3 beta, GSK3B

Entrez Gene IDs

2932 (Human)

Gene Symbol

GSK3B

UniProt

P49841

Additional GSK-3 beta Products

Product Documents for GSK-3 beta Antibody (3D10) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for GSK-3 beta Antibody (3D10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Apoptosis Intracellular Kinases

Carbohydrate Metabolism

Notch Pathway

Citations for GSK-3 beta Antibody (3D10) - BSA Free

Customer Reviews for GSK-3 beta Antibody (3D10) - BSA Free (4)

4.8 out of 5

4 Customer Ratings

5 Stars

75%

4 Stars

25%

3 Stars

2 Stars

1 Stars

Have you used GSK-3 beta Antibody (3D10) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Customer Images

Showing 1 - 4 of 4 reviews Showing All

Filter By:

GSK-3 beta Antibody (3D10)

Name: Natalie Erdmann

Application: Western Blot

Sample Tested: MM1S (multiple myeloma cell line)

Species: Human

Verified Customer | Posted 11/30/2016

Western blot analysis of GSK-3 beta antibody NBP1-47470 against MM1S cell lysate.

Conditions: - MM1S cells lysed with NP40 lysis buffer -protein quantified with BCA assay, 10 ug total protein loaded per lane -protein resolved by SDS-PAGE on 7.5% gel -transferred onto PVDF membrane and blocked with 5% BSA in TBST for 2hrs at room temperature -incubated with antibody (1:10,000) in TBST (+1% BSA) overnight at 4 deg with shaking -secondary incubation with HRP-conjugated anti-mouse antibody (1:10,000) for 1hr at room temperature -visualized with ECL chemilumnescence, imaged with film, exposure for 2 mins
GSK-3 beta Antibody (3D10)

Name: Anonymous

Application: Western Blot

Sample Tested: mouse beta cell line

Species: Mouse

Verified Customer | Posted 11/10/2016

western blot of Gsk3beta (1:1000) in mouse beta cell line (betaTC3) nuclear extract.
Name: Anonymous

Application: Simple Western

Sample Tested:

Species: Mouse

Verified Customer | Posted 08/05/2015

Simple western analysis of mouse brain tissue (striatum) from 4 month old Tg4150 and wildtype mice.
Name: Zachary Patinkin

Application: Simple Western

Sample Tested: Human mesenchymal stem cell whole cell lysate

Species: Human

Verified Customer | Posted 03/24/2015

Human mesenchymal stem cell whole cell lysate

There are no reviews that match your criteria.

Showing 1 - 4 of 4 reviews Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for GSK-3 beta Antibody (3D10) - BSA Free

Showing 1 - 2 of 2 FAQs Showing All

Q: I received this antibody in February 2012 and stored the unopened package immediately at 4C. Today (4/5/2012), I opened the package, placed the vial and centrifuged it briefly. I opened the top and found some thick beige-colored liquid which measured around 50 ul. I don't think I can use the antibody. Could you help me with the problem, please?

A: NBP1-47470 is supplied as ascitic fluid, which frequently has a beige-like color, and this could be what you're seeing in your vial. Otherwise, I'm concerned that the antibody may have been damaged by storing it a 4 C for such a long time. There is a very low concentration of preservative in this antibody, which is why we recommend storage at -20 C long term.
Q: Our customer would like to know whether NBP1-47470, GSK3 beta Antibody can be used in IF. It shows on the website that this antibody can be used to detect the target protein through ICC/IF. But his sampels are paraffin-embedded tissues, no cultured cells. Thus he is not sure. Please give us some advice and help.

A: Our GSK3 beta antibody with catalogue number NBP1-47470 has been validated for IHC with paraffin-embedded tissues, and has also been validated for immunocytochemistry. Our immunocytochemistry data can be seen in image 3 on our website, whilst our IHC-P data can be seen in image 4. This antibody is covered by our 100% quality guarantee for your customer's application of IHC with paraffin-embedded tissues. Currently tested species are human, mouse, rat and primate.
Q: I received this antibody in February 2012 and stored the unopened package immediately at 4C. Today (4/5/2012), I opened the package, placed the vial and centrifuged it briefly. I opened the top and found some thick beige-colored liquid which measured around 50 ul. I don't think I can use the antibody. Could you help me with the problem, please?

A: NBP1-47470 is supplied as ascitic fluid, which frequently has a beige-like color, and this could be what you're seeing in your vial. Otherwise, I'm concerned that the antibody may have been damaged by storing it a 4 C for such a long time. There is a very low concentration of preservative in this antibody, which is why we recommend storage at -20 C long term.
Q: Our customer would like to know whether NBP1-47470, GSK3 beta Antibody can be used in IF. It shows on the website that this antibody can be used to detect the target protein through ICC/IF. But his sampels are paraffin-embedded tissues, no cultured cells. Thus he is not sure. Please give us some advice and help.

A: Our GSK3 beta antibody with catalogue number NBP1-47470 has been validated for IHC with paraffin-embedded tissues, and has also been validated for immunocytochemistry. Our immunocytochemistry data can be seen in image 3 on our website, whilst our IHC-P data can be seen in image 4. This antibody is covered by our 100% quality guarantee for your customer's application of IHC with paraffin-embedded tissues. Currently tested species are human, mouse, rat and primate.