Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 736043
View all 17 beta-HSD14/HSD17B14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human 17 beta ‑HSD14/HSD17B14
Ala2-Ser270
Accession # Q9BPX1

Specificity

Detects human 17 beta ‑HSD14/HSD17B14 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human 17 beta-HSD14/HSD17B14 Antibody

17 beta -HSD14/HSD17B14 antibody in Human Brain by Immunohistochemistry (IHC-P).

17 beta ‑HSD14/HSD17B14 in Human Brain.

17 beta -HSD14/HSD17B14 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human 17 beta -HSD14/HSD17B14 Monoclonal Antibody (Catalog # MAB7515) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human 17 beta-HSD14/HSD17B14 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)

Reviewed Applications

Read 2 reviews rated 5 using MAB7515 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 17 beta-HSD14/HSD17B14

HSD-17b14 (hydroxysteroid (17-beta) dehydrogenase type14), also called 17bHSD14 or DHRS10, is a cytoplasmic 17beta-hydroxysteroid dehydrogenase that is a member of the SDR (short-chain dehydrogenase/reductase) family. It is a 270 amino acid, approximately 30 kDa protein with mRNA highly expressed in brain, placenta, liver and kidney. It converts estradiol to estrone, thus inactivating it, and is thought to regulate activity of estrogens and possibly dihydroepiandrosterone (DHEA) in the placenta and central nervous system. Human HSD-17b14 shares 80.5% amino acid sequence identity with mouse and rat HSD-17b14.

Long Name

17 beta Hydroxysteroid Dehydrogenase 14

Alternate Names

17 betaHSD14, DHRS10, HSD17B14, retSDR3, SDR3, SDR47C1

Entrez Gene IDs

51171 (Human); 66065 (Mouse); 691018 (Rat)

Gene Symbol

HSD17B14

UniProt

Q9BPX1 (Human)

Additional 17 beta-HSD14/HSD17B14 Products

Product Documents for Human 17 beta-HSD14/HSD17B14 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 17 beta-HSD14/HSD17B14 Antibody

For research use only

Customer Reviews for Human 17 beta-HSD14/HSD17B14 Antibody (2)

5 out of 5
2 Customer Ratings
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  • Human 17 beta-HSD14/HSD17B14 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 06/28/2022
    Human 17 beta-HSD14/HSD17B14 Antibody MAB7515
  • Human 17 beta-HSD14/HSD17B14 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Brain tissue
    Species: Human
    Verified Customer | Posted 12/31/2021
    Human 17 beta-HSD14/HSD17B14 Antibody MAB7515

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Protocols

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