Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1014838
View all 5-HT2B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human 5- HTR2B
Accession # P41595

Specificity

Detects human 5-HTR2B in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human 5-HT2B Antibody

5-HT2B antibody in Mouse Cerebellum by Immunohistochemistry (IHC-P).

5-HT2B in Mouse Cerebellum.

5-HT2B was detected in immersion fixed paraffin-embedded sections of mouse cerebellum using Mouse Anti-Human 5-HT2B Monoclonal Antibody (Catalog # MAB10322) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Purkinje neurons.

Applications for Human 5-HT2B Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse cerebellum

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5-HT2B

HTR2B is a G-protein coupled receptor for serotonin. The 5HT2 receptors mediate many of the central and peripheral physiologic functions of serotonin. 5HT2B plays a role in the regulation of dopamine and serotonin release, serotonin uptake and in the regulation of extracellular dopamine and serotonin levels. It also plays a role in pulmonary vasoconstriction and regulating cardiac structure and functions. 5HT2B receptors were shown to be expressed in human failing heart and have been strongly implicated in drug induced valvular heart disease.

Long Name

5-Hydroxytryptamine Receptor 2B

Alternate Names

5HT2B, HTR2B

Entrez Gene IDs

3357 (Human); 15559 (Mouse); 29581 (Rat)

Gene Symbol

HTR2B

UniProt

P41595

Additional 5-HT2B Products

Product Documents for Human 5-HT2B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human 5-HT2B Antibody

For research use only

Citations for Human 5-HT2B Antibody

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Protocols

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