Human 53BP1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB18772-100
MAB18772-SP
Detection  of Human 53BP1 by Western Blot.
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Human 53BP1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human 53BP1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1285C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human 53BP1
Ala1614-His1972
Accession # Q12888
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
1-25 µg/mL
See below
Immunocytochemistry
3-25 µg/mL
See below
Knockout Validated
53BP1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in 53BP1 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Human 53BP1 by Western Blot. View Larger

Detection of Human 53BP1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line and ZR‑75 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry 53BP1  in HeLa Human Cell Line. View Larger

53BP1 in HeLa Human Cell Line. 53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry 53BP1  in Human Cervical Cancer Tissue. View Larger

53BP1 in Human Cervical Cancer Tissue. 53BP1 was detected in immersion fixed paraffin-embedded sections of human cervical cancer tissue using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Knockout Validated Western Blot Shows Human 53BP1 Specificty Using Knockout Cell Line. View Larger

Western Blot Shows Human 53BP1 Specificty Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and 53BP1 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for 53BP1 at approximately 350 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockoutHeLa cell line. GAPDH (Catalog # MAB5718)is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated 53BP1  Specificity is Shown by Immunocytochemistry in Knockout Cell Line. View Larger

53BP1 Specificity is Shown by Immunocytochemistry in Knockout Cell Line. 53BP1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line but is not detected in 53BP1 knockout (KO) HeLa Human Cell Line cell line using Rabbit Anti-Human 53BP1 Monoclonal Antibody (Catalog # MAB18772) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 53BP1

The p53-binding protein 1 (53BP1) localizes to sites of damaged DNA and is phosphorylated by the ATM checkpoint kinase. 53BP1 is required for phosphorylation of other downstream ATM substrates, such as p53 and SMC-1, and therefore aids in nucleating a DNA damage response protein complex. Over aa 1614-1972, human 53BP1 shares 97% aa sequence identity with rat and mouse 53BP1. 

Long Name
p53 Binding Protein 1
Entrez Gene IDs
7158 (Human); 27223 (Mouse); 296099 (Rat)
Alternate Names
53BP1; MGC138366; p202; p53-binding protein 1; p53BP1,53BP1FLJ41424; TP53BP1; tumor protein 53-binding protein, 1; tumor protein p53 binding protein 1; tumor protein p53-binding protein, 1; tumor suppressor p53-binding protein 1

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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