Human A20/TNFAIP3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human A20/TNFAIP3 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 775928
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human A20/TNFAIP3
    Lys91-Leu263
    Accession # P21580
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
A20/TNFAIP3 in HL‑60 Human Cell Line. A20/TNFAIP3 was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Mouse Anti-Human A20/TNFAIP3 Monoclonal Antibody (Catalog # MAB75981) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: A20/TNFAIP3
A20, also called TNF alpha -induced protein 3 (TNFAIP3), is a cytoplasmic zinc finger protein that inhibits NF kappa B activity and tumor necrosis factor-mediated programmed cell death. The protein interacts with NAF1 and inhibits TNF-induced NF kappa B-dependent gene expression by interfering with RIP- or TRAF2-mediated transactivation signaling. A20 contains an N-terminal domain which has deubiquitinating enzyme activity and removes ubiquitin chains from receptor-interacting protein (RIP), thus mediating distinct regulatory effects in the down-regulation of NF kappa B signaling.
  • Entrez Gene IDs:
    7128 (Human); 21929 (Mouse)
  • Alternate Names:
    A20; A20TNFA1P2; EC 3.4.19.12; EC 6.3.2.-; MGC104522; MGC138687; OTU domain-containing protein 7C; OTUD7C; OTUD7CMGC138688; Putative DNA-binding protein A20; TNF alpha-induced protein 3; TNFA1P2; TNFAIP3; tumor necrosis factor alpha-induced protein 3; tumor necrosis factor inducible protein A20; tumor necrosis factor, alpha-induced protein 3; Zinc finger protein A20
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