Human ADAM9 Ectodomain Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human ADAM9 Ectodomain in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant mouse (rm) rmADAM9 is observed and less than 0.05% cross-reactivity with recombinant human (rh) rhADAM8, rhADAM10, rhTACE, rhTIMP-1, rhTIMP-2, rhTIMP-3, rhTIMP-4, rhBACE-1 and rmADAM10 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human ADAM9
    Ala206-Asp697
    Accession # Q13443
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunoprecipitation
    25 µg/mL
    Conditioned cell culture medium spiked with Recombinant Human ADAM9 (Catalog # 939‑AD), see our available Western blot detection antibodies
    • Human ADAM9 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Human ADAM9 Ectodomain Antibody (Catalog # AF939)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Human ADAM9 Ectodomain Biotinylated Antibody (Catalog # BAF939)
  • ELISA Standard
     
    Recombinant Human ADAM9 Protein, CF (Catalog # 939-AD)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ADAM9 by Western Blot. Western blot shows lysates of WI‑38 human lung fibroblast cell line and U‑87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ADAM9 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF939) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for ADAM9 at approximately 110 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM9

ADAM9, also known as MDC9 or meltrin gamma, is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain (1). Like other membrane-anchored ADAMs, ADAM9 consists of a pro domain with a cysteine switch and furin cleavage sequence, a catalytic domain with the zinc-binding site and Met-turn expected for reprolysins, a disintegrin-like domain, a cysteine-rich domain, an EGF-like domain, a transmembrane domain, and the cytoplasmic domain. ADAM9 is able to cleave peptides corresponding to cleavage sites of tumor necrosis factor-alpha (TNF-alpha ), the p75 TNF receptor, the beta -amyloid protein precursor, and the c-kit ligand-1, implying that it may participate in shedding of these membrane proteins (2). In fact, ADAM9 has been shown to shed membrane-anchored heparin-binding EGF-like growth factor (3). In addition, it also cleaves oxidized insulin beta -chain and fibronectin (2,4). Besides its catalytic activity, ADAM9 functions as an adhesion molecule through binding of its disintegrin domain to integrins such as alpha v beta 5 and alpha 6 beta 1 (5, 6). The cytoplasmic domain of ADAM9 interacts with Src homology 3 (SH3)‑containing proteins and protein kinase C, and may mediate different signaling pathways (3, 7). ADAM9 is widely expressed in tissues (8).

  • References:
    1. Moss, et al. (2001) DDT 6:417.
    2. Roghan, et al. (1999) J. Biol. Chem. 274:3531.
    3. Izumi, et al. (1998) EMBO J. 17:7260.
    4. Schwettmann and Tschesche (2001) Prot. Expre. & Purif. 21:65.
    5. Nath, et al. (2000) J. Cell Sci. 113:2319.
    6. Zhou, et al. (2001) Biochem. Biophys. Res. Comm. 280:574.
    7. Howard, et al. (1999) J. Biol. Chem. 274:31693.
    8. Weskamp, et al. (1996) J. Cell. Biol. 132:717.
  • Long Name:
    A Disintegrin and Metalloprotease-like Domain 9
  • Entrez Gene IDs:
    8754 (Human); 11502 (Mouse)
  • Alternate Names:
    a disintegrin and metalloproteinase domain 9 (meltrin gamma); ADAM 9; ADAM metallopeptidase domain 9 (meltrin gamma); ADAM metallopeptidase domain 9; ADAM9; Cellular disintegrin-related protein; cone rod dystrophy 9; CORD9; disintegrin and metalloproteinase domain-containing protein 9; EC 3.4.24; EC 3.4.24.-; MCMP; MCMPMDC9KIAA0021Mltng; MDC9; Meltrin gamma; Meltrin-gamma; Metalloprotease/disintegrin/cysteine-rich protein 9; MLTNG; Myeloma cell metalloproteinase
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Expression profiles and clinical correlations of degradome components in the tumor microenvironment of head and neck squamous cell carcinoma.
    Authors: Stokes A, Joutsa J, Ala-Aho R, Pitchers M, Pennington CJ, Martin C, Premachandra DJ, Okada Y, Peltonen J, Grenman R, James HA, Edwards DR, Kahari VM
    Clin. Cancer Res., 2010;16(0):2022.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Role of ADAM-9 disintegrin-cysteine-rich domains in human keratinocyte migration.
    Authors: Zigrino P, Steiger J, Fox JW, Loffek S, Schild A, Nischt R, Mauch C
    J. Biol. Chem., 2007;282(42):30785-93.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Transmembrane collagen XVII, an epithelial adhesion protein, is shed from the cell surface by ADAMs.
    Authors: Franzke CW, Tasanen K, Schacke H, Tryggvason K, Mauch C, Zigrino P, Sunnarborg S, Fahrenholz F, Bruckner-Tuderman L
    EMBO J., 2002;21(19):5026-35.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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