Human AIF-1/Iba1 Antibody

Catalog # Availability Size / Price Qty
MAB7308
MAB7308-SP
AIF-1/Iba1 in Human Spleen.
1 Image
Product Details
FAQs
Supplemental Products
Reviews (2)

Human AIF-1/Iba1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human AIF-1/Iba1 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 603102
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human AIF-1/Iba1
Met1-Pro147
Accession # P55008
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
0.5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry AIF-1/Iba1 antibody in Human Spleen by Immunohistochemistry (IHC-P). View Larger

AIF-1/Iba1 in Human Spleen. AIF-1/Iba1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIF-1/Iba1 Monoclonal Antibody (Catalog # MAB7308) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AIF-1/Iba1

Allograft-inflammatory factor 1 (AIF-1), also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed in activated macrophages and is important for the proinflammatory response to ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular smooth muscle and endothelial cells in these areas where it promotes vascular remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue during transplant rejection. It also promotes the proliferation of breast cancer cells and in vivo tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the N-terminal 54 aa of isoform 3. Isoform 2 lacks
aa 1-54 as well as aa 121-147 but also has a 66 aa insertion at position 65.

Long Name
Allograft Inflammatory Factor 1
Entrez Gene IDs
199 (Human); 11629 (Mouse); 29427 (Rat)
Alternate Names
AIF1; AIF-1; allograft inflammatory factor 1; G1; IBA1; IbaI; interferon gamma responsive transcript; Ionized calcium-binding adapter molecule 1; IRT1; IRT-1; Protein G1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human AIF-1/Iba1 Antibody

Average Rating: 5 (Based on 2 Reviews)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human AIF-1/Iba1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human AIF-1/Iba1 Antibody
By Anonymous on 05/01/2022
Application: ICC/IF Sample Tested: Adrenal gland tissue Species: Human

1:1000 dilution is good


Human AIF-1/Iba1 Antibody
By Anonymous on 12/29/2021
Application: IHC Sample Tested: Spleen tissue Species: Human