AIF-1/Iba1 in Human Spleen. AIF-1/Iba1 was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human AIF-1/Iba1 Monoclonal Antibody (Catalog # MAB7308) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Allograft-inflammatory factor 1 (AIF-1),
also known as daintain and Iba1, is a 17 kDa cytoplasmic protein that contains
two calcium-binding EF hand motifs (aa 45-80 and aa 81-115). AIF-1 is expressed
in activated macrophages and is important for the proinflammatory response to
ischemia, tissue injury, and atherosclerosis. It is also expressed in vascular
smooth muscle and endothelial cells in these areas where it promotes vascular
remodeling and new vessel formation. AIF-1 is upregulated in allografted tissue
during transplant rejection. It also promotes the proliferation of breast
cancer cells and in vivo
tumorigenesis. The 147 amino acid (aa) isoform 3 of human AIF-1 shares 89% aa
sequence identity with mouse and rat AIF-1. Isoform 1 of human AIF-1 lacks the
N-terminal 54 aa of isoform 3. Isoform 2 lacks aa 1-54 as well as aa 121-147
but also has a 66 aa insertion at position 65.
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