Detection of Human alpha 1‑Acid Glycoprotein by Western Blot. Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human alpha 1‑Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for alpha 1‑Acid Glycoprotein at approximately 40-50 kDa (as indicated). This experiment was conducted under reducing and non-reducing conditions and using Immunoblot Buffer Group 1.
alpha 1-Acid Glycoprotein in Human Liver. alpha 1-Acid Glycoprotein was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human alpha 1-Acid Glycoprotein Monoclonal Antibody (Catalog # MAB3694) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 1-Acid Glycoprotein
alpha 1-Acid Glycoprotein (AGP-1), also known as orosomucoid-1, is an acute phase protein secreted by the liver. Serum AGP-1 levels are elevated during inflammatory responses. AGP-1 binds a wide range of molecules in the circulation. Human and mouse AGP-1 share 43% amino acid sequence identity.
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